N3162

Anti-NBS1 (Nibrin) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Sinónimos: Anti-p95 Protein of the MRE11/RAD50 Complex
• Número MDL: MFCD07784851
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• conjugado: unconjugated
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• formulario: buffered aqueous solution
• mol peso: antigen 95 kDa (also recognizes 100 kDa band)
• reactividad de especies: canine, human, rat, chimpanzee
• técnicas: immunoprecipitation (IP): 5-10 μg; indirect immunofluorescence: 5-10 μg/mL using Hela cells, fixed with paraformaldehyde/triton; microarray: suitable; western blot: 1-2 μg/mL using MCF7 nuclear extracts
• Nº de acceso UniProt: O60934
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... NBN(4683) ; mouse ... Nbn(27354) ; rat ... Nbn(85482)

Descripción

Descripción general

NBS1 (Nibrin), also known as p95 protein of the meiotic recombination 11 homolog 1/DNA repair protein RAD50 (MRE11/RAD50) complex. NBS1 contains two domains found in the cell cycle checkpoint proteins forkhead-associated domain (FHA) and an adjacent breast cancer carboxyterminal domain (BRCT). NBS1 is one of the protein components of the double-strand break repair complex NBS1/MRE11/p50.

Nibrin (NBS1) is made up of 754 amino acids.

Inmunógeno

synthetic peptide corresponding to amino acids 692-706 of mouse NBS1 (nibrin), conjugated to KLH via an N-terminal added cysteine residue.

Aplicación

Anti-NBS1 (Nibrin) antibody has been used:

• in immunofluorence
• in immunoblotting
• in immunoprecipitation

Acciones bioquímicas o fisiológicas

NBS1 (Nibrin) was first isolated as a protein involved in DNA repair through analysis of mutations in patients with Nijmegen breakage syndrome (NBS). The majority of NBS patients bear a five base pairs deletion that leads to truncated NBS1, called 657del5. p95/NBS1 (nibrin) deficiency abrogates the formation of the MRE11/RAD50 ionizing radiation-induced foci, revealing a molecular link between DSB repair and cell cycle checkpoints activated by DNA damage.

Nibrin (NBS1) is involved in stabilizing genomes and has a role in development of cancers. It also takes part in the repair mechanism after double strand breaks in the DNA.

Forma física

Solution in 0.01 M phosphate buffered saline containing 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable