N0913

Anti-Neurocan antibody, Mouse monoclonal

clone 650.24, purified from hybridoma cell culture

• Sinónimos: Anti-CSPG3, Anti-Chondroitin Sulfate Proteogycan 3
• Número MDL: MFCD02263808
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 200
• conjugado: unconjugated
• forma del anticuerpo: purified from hybridoma cell culture
• tipo de anticuerpo: primary antibodies
• clon: 650.24, monoclonal
• Formulario: buffered aqueous solution
• mol peso: antigen 260 kDa (additional band at 160 kDa)
• reactividad de especies: rat
• envase: antibody small pack of 25 μL
• concentración: ~1 mg/mL
• técnicas: immunocytochemistry: suitable; immunohistochemistry (frozen sections): suitable; indirect ELISA: suitable; western blot: 0.1-0.2 μg/mL using neonatal rat brain treated with chondroitinase.
• isotipo: IgG1
• Nº de acceso UniProt: P55067
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: rat ... Ncan(58982)

Descripción

Descripción general

Monoclonal Anti-Neurocan (mouse IgG1 isotype) is derived from the hybridoma 650.24 produced by the fusion of mouse myeloma cells (P3X cells) and splenocytes from BALB/c mice immunized with embryonic rat brain proteoglycans. Neurocan is a chondroitin sulfate proteoglycan (known also as CSPG3). It accounts for 20−30% of chondroitin sulfate proteoglycans in early postnatal rat brain. The mouse neurocan cDNA encodes for a 1,257-amino acid protein with a 22-amino acid signal peptide followed by an immunoglobulin-like domain and repeating motifs characteristic of the hyaluronic acid-binding region of aggregating proteoglycans. Neurocan is found in large amounts in glial scar, a primarily astrocytic structure formed after an injury in the central nervous system (CNS).

Neurocan is a proteoglycan which is expressed in the nervous tissue.

Inmunógeno

embryonic rat brain proteoglycans.

Aplicación

Anti-Neurocan antibody, Mouse monoclonal has been used in:

• western blotting
• immunofluorescence
• enzyme-linked immunosorbent assay (ELISA)
• immunocytochemistry
• immunohistochemistry

Acciones bioquímicas o fisiológicas

Neurocan can inhibit the growth of axons. In presence of central nervous system injuries, it also takes part in tissue repair and neural network reconstruction.

Neurocan participates in the modulation of cell adhesion and migration. It exerts a negative effect on growing cerebellar axons.

Descripción de destino

Neurocan is a chondroitin sulfate proteoglycan (knownalso as CSPG3) thought to be involved in the modulation of cell adhesion and migration. It accounts for 20-30% of chondroitin sulfate proteoglycans in early postnatal rat brain.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable