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MWND500

Sigma-Aldrich

Kit for Molecular Weights 14,000-500,000 Non-denaturing

Sinónimos:

protein markers, protein molecular weight standards, protein standards

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About This Item

Código UNSPSC:
41105337
NACRES:
NA.32

formulario

lyophilized powder

temp. de almacenamiento

−20°C

Categorías relacionadas

Aplicación

Kit for Molecular Weights 14,000-500,000 Non-denaturing has been used as a standard for non-denaturing PAGE (polyacrylamide gel electrophoresis) analysis. It has also been used to calibrate columns for gel exclusion chromatography.

Envase

The kit contains 5 vials of proteins (1 mg of each) having a molecular weight range of 14 - 545 kDa and a technical bulletin.

Proteins:
α-Lactalbumin from bovine milk
Carbonic Anhydrase from bovine erythrocytes
Albumin from chicken egg white
Albumin from bovine serum
Urease from Jack bean

Reconstitución

Reconstitute the contents of each vial of protein marker with 1ml of water, with the exception of Urease, Catalog Number U7752. Reconstitute the vial of Urease with 0.5 ml of water and mix until the protein is in solution. Then add 0.5 ml of glycerol to the Urease solution to stabilize the protein during freeze thaw cycles. Solutions may be stored at -20 °C for future use. It is recommended that repeated freezing and thawing be avoided. Stock solutions may be dispensed into working aliquots, frozen, and then discarded after 2-3 uses. Immediately before use, thaw an aliquot of each prepared protein marker and dilute with an equal volume of sample buffer.

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Referencia del producto
Descripción
Precios

Pictogramas

Health hazardExclamation mark
GHS08,GHS07

Palabra de señalización

Danger

Frases de peligro

H315,H319,H334,H335

Clasificaciones de peligro

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Órganos de actuación

Respiratory system

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Assembly of phagocyte NADPH oxidase: A concerted binding process?
Gilda Karimi
Biochim. Biophys. Acta Gen. Subj. (2014)
Immunogenicity and Serological Cross-Reactivity of Saliva Proteins among Different Tsetse Species
Xin Zhao
PLoS ONE, e0004038-e0004038 (2015)
Oligomerization of the microtubule-associated protein tau is mediated by its N-terminal sequences: implications for normal and pathological tau action
H. Eric Feinstein
Journal of Neurochemistry (2016)
Gilda Karimi et al.
Biochimica et biophysica acta, 1840(11), 3277-3283 (2014-08-12)
The phagocyte NADPH-oxidase is a multicomponent enzyme that generates superoxide anions. It comprises a membrane redox component flavocytochrome b558 and four cytosolic proteins (p67(phox), p47(phox), p40(phox) and Rac) that must assemble to produce an active system. In this work we

Protocolos

HPLC Analysis of Various Cationic Polymers on TSKgel® PWXL-CP Columns

Separation of Polyacrylic acid (PAA) 438 kDa; Polyacrylic acid (PAA) 235 kDa; Polyethylenimine (PEI) 266 kDa ; Poly(dimethyl diallyl ammonium chloride) (PIDADMACl) 204 kDa; p-Aminosalicylic acid (PAS) 7800 Da; p-Aminosalicylic acid (PAS) 257 kDa; Cationic dextran (11 kDa); Chitosan (13.4 kDa)

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