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MTOX1105

Sigma-Aldrich

MRP3 Knockout HepaRG™ Cells

human female liver (hepatocarcinoma and hepatitis C)

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About This Item

Código UNSPSC:
12352207
NACRES:
NA.81

Nombre del producto

MRP3 Knockout HepaRG™ Cells, 1 vial

origen biológico

human female liver (hepatocarcinoma and hepatitis C)

Nivel de calidad

200

Nº de acceso OMIM

604323

temp. de almacenamiento

−196°C

Información sobre el gen

human ... MRP3(8714)

Descripción general

HepaRG is a human hepatoma cell line. The cells possess a pseudodiploid karyotype and have been characterized as an oval ductular bipotent hepatic cell line as they have the ability to differentiate into both biliary and hepatocyte lineages in the presence of DMSO. HepaRG MRP3 knockout cells express the major xenobiotic sensors (PXR, CAR and AhR), drug transporters, phase I and II drug metabolizing enzymes as well as key hepatic transcription factors involved in stress response pathways.

Aplicación

See technical bulletin for detailed protocols

Características y beneficios

HepaRG cells are the most metabolically active human hepatocyte cell line developed to date. These the cells are suitable for a wide variety of studies for drug metabolism, CYP induction, metabolism-mediated toxicity, transporter, and hepatotoxicity.

  • Sigma′s HepaRG MRP3 Knockout (KO) allows investigations of drug-transporter interactions involving MRP3 in the liver.
Zinc finger nucleases (ZFN) mediated Knock-out of ABCC3 (MRP3) gene.

  • The frame-shift mutation of ABCC3 gene was confirmed by fragment length analysis and DNA sequencing.
  • Loss of functionality was confirmed by loss of transport of selective substrates in sandwich culture assay.

Calidad

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation.

Información legal

These products are covered by the License Agreement as described in Exhibit 1 and 2, in the technical bulletin.
HepaRG is a trademark of BioPredic International company

Cláusula de descargo de responsabilidad

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

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Artículos

The Role of Liver Transporters in Drug-Drug Interactions

Oral drug delivery involves dissolution in the small intestine and absorption across the enterocyte barrier into the portal vein followed by subsequent delivery through the liver into the systemic circulation.

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico