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MP0040A

Sigma-Aldrich

LookOut® Mycoplasma qPCR Detection Kit

Sinónimos:

Quantiative PCR mycoplasma detection kit

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About This Item

Número CE:
200-664-3
Código UNSPSC:
12352207
NACRES:
NA.75

uso

sufficient for 25 reactions

Nivel de calidad

200

calidad

Not suitable for clinical diagnostic use.

envase

pkg of 1 kit

condiciones de almacenamiento

dry at room temperature

técnicas

PCR: suitable

aplicaciones

detection
microbiology

temp. de almacenamiento

2-8°C

Categorías relacionadas

Descripción general

The LookOut Mycoplasma PCR Detection Kit utilizes the polymerase chain reaction (PCR), which is established as the method of choice for highest sensitivity in the detection of Mycoplasma and Acholeplasma contamination in cell cultures and other cell culture derived biologicals.

The primer/probe system detects the highly conserved 23S rRNA operon coding region of the mycoplasma genome. The kit is highly specific and does not detect eukaryotic DNA. The detection spectrum includes most mycoplasma species identified as cell culture contaminants (see Table 2 in the technical bulletin). The kit contains the nucleotide dUTP instead of dTTP and is, therefore, suitable for UNG pretreatment.

This kit already contains Taq polymerase in the Mycoplasma Mix.

Aplicación

qPCR Probe Kit for high quality and reliable quantitative detection of mycoplasma DNA in research, industrial application and product testing. Usable for direct testing of cell cultures and biologicals in combination with cell culture enrichment.

Información legal

LookOut is a registered trademark of Merck KGaA, Darmstadt, Germany

Código de clase de almacenamiento

10 - Combustible liquids

Punto de inflamabilidad (°F)

188.6 °F

Punto de inflamabilidad (°C)

87 °C

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Artículos

Why Use Antibiotics in Cell Culture?

Know when to use antibiotics to prevent bacterial or fungal, mycoplasma, or viral contamination in cell culture and find suitable antibiotics or other biological agents.

Mycoplasma Detection and Elimination in Cell Culture

Detect mycoplasma contamination in cell culture through the PCR, DNA stain, or culture tests. Discover mycoplasma prevention, elimination, and detection kits.

Fetal Bovine Serum (FBS) – What You Need to Know

Review the basics of using fetal bovine (or calf) serum for cell culture and how sourcing and regulations affect the price of sera for cell-based assays.

Protocolos

Optimized PCR-based Detection of Mycoplasma

Mycoplasma contamination of cell cultures is a serious issue impacting cell model validity. PCR testing for mycoplasma is an inexpensive, sensitive, and specific method for detecting contamination.

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