MAK508

Choline Assay Kit

sufficient for 100 colorimetric or fluorometric tests

• Código UNSPSC: 12161503
• NACRES: NA.84

Propiedades

• Nivel de calidad: 100
• aplicaciones: food and beverages; pharmaceutical
• método de detección: colorimetric; fluorometric
• enfermedades relevantes: anxiety; neurological disorders
• Condiciones de envío: wet ice
• temp. de almacenamiento: −20°C

Descripción

Descripción general

Choline and its metabolites play important roles in membrane structure integrity, cellular signaling and cholinergic neurotransmission. Aberrant regulation in choline metabolism has been associated with mental illness such as anxiety.

Características y beneficios

• Linear detection range: 1 to 100 µM for the colorimetric assay and 0.2 to 10 µM for the fluorometric assay.

Idoneidad

The kit is suitable for the determination of choline in biological samples such as serum, plasma, urine, saliva, milk, tissue, and cell culture, as well as for studying the effects of drugs on choline metabolism.

Principio

The Choline Assay Kit method provides a simple, direct and high-throughput assay for measuring choline in biological samples. In this assay, free choline is oxidized by choline oxidase to betaine and H2O2 which reacts with a specific dye to form a pink colored product. The color intensity at 570 nm or fluorescence intensity λEx = 530 nm/λEm = 585 nm is directly proportional to the choline concentration in the sample.

Información de seguridad

• Pictogramas: GHS08
• Palabra de señalización: Danger
• Frases de peligro: H334,H412
• Consejos de prudencia: P261 - P273 - P284 - P501
• Clasificaciones de peligro: Aquatic Chronic 3 - Resp. Sens. 1
• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 3