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MAK051

Sigma-Aldrich

Aconitase Activity Assay Kit

sufficient for 100 colorimetric tests

Sinónimos:

Aconitase Enzyme Activity Kit

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About This Item

Código UNSPSC:
12161503
NACRES:
NA.84

uso

sufficient for 100 colorimetric tests

método de detección

colorimetric

enfermedades relevantes

neurological disorders; cardiovascular diseases; cancer

temp. de almacenamiento

2-8°C

Información sobre el gen

human ... ACO1(48) , ACO2(50)
mouse ... ACO1(11428) , ACO2(11429)
rat ... ACO1(50655) , ACO2(79250)

Descripción general

The aconitases are a family of iron-sulfur containing enzymes that catalyzes the isomerization of citrate to isocitrate. Two aconitases, encoded by different genes, have been identified in eukaryotes. Aconitase 1 (ACO1, c-aconitase) is a cytosolic enzyme while Aconitase 2 (ACO2, m-aconitase) is a mitochondrial enzyme that functions in the tricarboxylic acid cycle. Aconitases are reversibly inactivated by oxidative stress, and aconitase activity in cells and tissues has been used as a biomarker for oxidative damage.

Aplicación

Aconitase Activity Assay Kit has been used to measure the activity of cytosolic and mitochondrial aconitase enzyme.

Características y beneficios

Compatible with high-throughput handling systems.

Idoneidad

Suitable for the measurement of aconitase activity in biological samples including tissue and cells

Principio

The Aconitase Activity Assay kit provides a simple and direct procedure for measuring Aconitase activity in a variety of samples. Aconitase activity is determined in a coupled enzyme reaction in which citrate is converted to isocitrate by aconitase. This results in a colorimetric (450 nm) product proportional to the enzymatic activity present. One unit of aconitase is the amount of enzyme that will isomerize 1.0 μmole of citrate from isocitrate per minute at pH 7.4 at 25 °C.

sustituido por

Referencia del producto
Descripción
Precios

Pictogramas

Health hazardCorrosion
GHS08,GHS05

Palabra de señalización

Danger

Frases de peligro

H314,H334

Clasificaciones de peligro

Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B

Código de clase de almacenamiento

8A - Combustible corrosive hazardous materials

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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(+)-Potassium Ds-threo-isocitrate monobasic ≥98.0% (NT)

Sigma-Aldrich

58790

(+)-Potassium Ds-threo-isocitrate monobasic

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