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Merck

M8537

Sigma-Aldrich

MCDB 131 Medium

With trace elements and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Sinónimos:

Molecular, Cellular, and Development Biology 131 medium

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About This Item

Código UNSPSC:
12352207
NACRES:
NA.75

formulario

powder

concentración

11.6 g/L

técnicas

cell culture | mammalian: suitable

componentes

sodium pyruvate: 0.11 g/L
phenol red: 0.012 g/L
L-glutamine: 1.461 g/L
HEPES: no
glucose: 1.0 g/L (Dextro)

Condiciones de envío

ambient

temp. de almacenamiento

2-8°C

Descripción general

MCDB-131 medium provides a defined and optimally balanced nutritional environment that selectively promotes the growth of specific cell types. MCDB-131 is designed to support clonal growth of human microvascular endothelial cells, including human umbilical vein endothelial cells, when supplemented with 0.7% FBSP, 10 ng/ml epidermal growth factor, and 1 μg/ml hydrocortisone. MCDB media is made for the low-protein or serum-free growth of specific cell types using hormones, growth factors, trace elements, or low levels of dialyzed fetal bovine serum protein (FBSP). 

Aplicación

MCDB-131 Medium has been used:

  • as a component of the basal medium to culture bovine retinal endothelial cells
  • to culture bovine lung microvascular endothelial cells
  • for the isolation of porcine endocranial endothelial cells and porcine aortic endothelial cells

Cantidad

Formulated to contain 11.6 grams of powder per liter of medium.

Reconstitución

Supplement with 1.18 g/L sodium bicarbonate.

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Protocolos

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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