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Merck

M8318

Sigma-Aldrich

Anti-AtMPK3 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Sinónimos:

Anti-Arabidopsis thaliana MAP Kinase 3

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About This Item

Número MDL:
Código UNSPSC:
12352203
NACRES:
NA.44

origen biológico

rabbit

Nivel de calidad

conjugado

unconjugated

forma del anticuerpo

affinity isolated antibody

tipo de anticuerpo

primary antibodies

clon

polyclonal

Formulario

buffered aqueous solution

mol peso

antigen 43 kDa

reactividad de especies

Arabidopsis thaliana

técnicas

western blot: 1-2 μg/mL using extract (cytosolic fraction) of Arabidopsis thaliana leaves
western blot: 2-4 μg/mL using extract (cytosolic fraction) of Nicotiana tabacum leaves

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

modificación del objetivo postraduccional

unmodified

Información sobre el gen

Arabidopsis thaliana ... ATMPK3(823706)

Categorías relacionadas

Descripción general

The protein kinase AtMPK3 is a component of the MAP kinase cascade.

Especificidad

Anti-AtMPK3 recognizes AtMPK3.

Inmunógeno

Synthetic peptide corresponding to amino acids at the C-terminus of Arabidopsis thaliana MPK3. This sequence is specific to AtMPK3 (not found in the other 20 known AtMPK isoforms). It has limited homology (58% identity) to the AtMPK6 isoform and shares significant homology (67% identity, continuous epitope) with the Nicotiana tabacum ortholog WIPK.

Aplicación

Anti-AtMPK3 antibody produced in rabbit has been used in immunoblotting analysis.

Acciones bioquímicas o fisiológicas

AtMPK3 is a component of the plant MAPK signaling cascade that functions downstream of the FLS2 receptor and regulates the innate response to bacterial and fungal infections. AtMPK3 is also involved in the phosphorylation of VIP1 in response to Agrobacterium infection and the subsequent transfer of the bacterial T-DNA into the nucleus. RNAi mediated inhibition of AtMPK6 causes prolonged activation of AtMPK3 . Anti-AtMPK3 antibody is specific for AtMPK3 (43 kDa). The product also reacts with Nicotiana tabacum and p56 in Papaver rhoeas . In immunoblotting, staining of AtMPK3 is specifically inhibited with AtMPK3 immunizing peptide (Arabidopsis thaliana, amino acids 359-370).

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Almacenamiento y estabilidad

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Producto relacionado

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

nwg

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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Armin Djamei et al.
Science (New York, N.Y.), 318(5849), 453-456 (2007-10-20)
Nuclear import of transfer DNA (T-DNA) is a central event in Agrobacterium transformation of plant cells and is thought to occur by the hijacking of certain host cell proteins. The T-DNA-associated virulence protein VirE2 mediates this process by binding to
Regulation of MAPKs by growth factors and receptor tyrosine kinases
Katz M, et al.
Biochimica et Biophysica Acta - Molecular Cell Research, 1773(8) (2007)
Expression pattern and core region analysis of AtMPK3 promoter in response to environmental stresses
Gao F, et al.
Science China: Life Sciences, 53(11), 1315-1321 (2010)
Different protein kinase families are activated by osmotic stresses in Arabidopsis thaliana cell suspensions
Droillard MJ, et al.
Febs Letters, 527(1-3) (2002)
CBL-interacting protein kinase 6 negatively regulates immune response to Pseudomonas syringae in Arabidopsis
Sardar A, et al.
Journal of Experimental Botany, 68(13), 3573-3584 (2017)

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