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Merck

M7939

Sigma-Aldrich

Anti-Myosin IIB, non-muscle antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Sinónimos:

Anti-Myosin-10

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About This Item

Número MDL:
Código UNSPSC:
12352203
NACRES:
NA.41

origen biológico

rabbit

Nivel de calidad

conjugado

unconjugated

forma del anticuerpo

affinity isolated antibody

tipo de anticuerpo

primary antibodies

clon

polyclonal

Formulario

buffered aqueous solution

mol peso

antigen ~200 kDa

reactividad de especies

rat, human, canine

técnicas

western blot (chemiluminescent): 1:200 using whole cell extracts of cultured dog MDCK kidney cells, cultured rat NRK cells, and cultured human Jurkat cells.

Nº de acceso UniProt

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

modificación del objetivo postraduccional

unmodified

Información sobre el gen

Descripción general

Myosin IIB (nonmuscle) is a relatively abundant, widespread two headed myosin composed of an N-terminal motor domain, a light chain binding neck region, a coiled-coil region, and a nonhelical C-terminal domain. It forms a heterohexamer composed of a pair of heavy chains and two pairs of light chains. Myosin IIB is expressed in embryonic cardiac myocytes.

Especificidad

Anti-Myosin IIB, non-muscle antibody is specific for the heavy chain of human myosin IIB (nonmuscle). The antibody also reacts with myosin in dogs and rats.

Inmunógeno

Synthetic peptide corresponding to amino acid residues 1965-1976 of the heavy chain of human myosin IIB (nonmuscle) conjugated to KLH. This sequence is identical in rabbit and differs by one and two amino acids from the respective bovine and rat sequences.

Aplicación

Anti-Myosin IIB, non-muscle antibody produced in rabbit has been used in:
  • immunoblotting
  • stimulated emission depletion (STED) nanoscopy
  • immunofluorescence

Acciones bioquímicas o fisiológicas

Myosin IIB plays a key role in the COS-7 cell cytokinesis and is need for the initiation of ring constriction.
Myosins are actin-dependent motors that have 15 classes based on their phylogenetics . Myosin IIb regulates memory and synaptic plasticity functions. Nonmuscle myosin IIb has been implicated in fibroblast movement and erythroblast enucleation .

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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Visite la Librería de documentos

Non-muscle myosin heavy chain IIA and IIB interact and co-localize in living cells: relevance for MYH9-related disease
Marini M, et al.
International Journal of Molecular Medicine, 17(5), 729-736 (2006)
Life without double-headed non-muscle myosin II motor proteins
Betapudi V
Frontiers in Chemistry, 2, 45-45 (2014)
Localization of sarcomeric proteins during myofibril assembly in cultured mouse primary skeletal myotubes
White J, et al.
The Anatomical Record, 297(9), 1571-1584 (2014)
The role of pulmonary vascular contractile protein expression in pulmonary arterial hypertension
Konik EA, et al.
Journal of Molecular and Cellular Cardiology, 65(12), 147-155 (2013)
Myosin II recruitment during cytokinesis independent of centralspindlin-mediated phosphorylation
Beach JR and Egelhoff TT
Test, 284(40), 27377-27383 (2009)

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