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M7278

Sigma-Aldrich

Medio esencial mínimo Eagle

HEPES modification, with Earle′s salts and sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Sinónimos:

MEM

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About This Item

Código UNSPSC:
12352207
NACRES:
NA.75

product name

Medio esencial mínimo Eagle, HEPES Modification, with Earle′s salts, 25 mM HEPES and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Nivel de calidad

400

esterilidad

sterile-filtered

formulario

liquid

técnicas

cell culture | mammalian: suitable

impurezas

endotoxin, tested

pH

>7.2

componentes

L-glutamine: no
phenol red: yes
HEPES: 25 mM
sodium pyruvate: no
Earle’s salts (5% CO2): yes
NaHCO3: yes

Condiciones de envío

ambient

temp. de almacenamiento

2-8°C

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Descripción general

Minimum Essential Medium Eagle comprises sugar, inorganic salts, water-soluble vitamins and essential amino acids. It is widely used as a media formulation for many cell culture studies.

Aplicación

Minimum Essential Medium Eagle has been used for culturing primordial follicles and ovary tissues, human skin fibroblast cells, human colonic epithelial cell line LS174T and hippocampal tissue slices.

Reconstitución

Supplement with 0.292 g/L L-glutamine.

Otras notas

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

suplemento

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Shafagh A Waters et al.
The EMBO journal, 36(3), 374-387 (2016-11-12)
RNA sequencing studies have identified hundreds of non-coding RNAs in bacteria, including regulatory small RNA (sRNA). However, our understanding of sRNA function has lagged behind their identification due to a lack of tools for the high-throughput analysis of RNA-RNA interactions
Rodrigo Lerchundi et al.
Frontiers in cellular neuroscience, 14, 80-80 (2020-05-07)
Genetically encoded nanosensors such as the FRET-based adenosine triphosphate (ATP) sensor ATeam enable the measurement of changes in ATP levels inside cells, promoting our understanding of metabolic interactions between astrocytes and neurons. The sensors are usually well characterized in vitro
Alfred K Yu et al.
Human molecular genetics, 24(10), 2848-2860 (2015-02-06)
Mitochondrial complex I (NADH dehydrogenase) is a major contributor to neuronal energetics, and mutations in complex I lead to vision loss. Functional, neuroanatomical and transcriptional consequences of complex I deficiency were investigated in retinas of the Ndufs4 knockout mouse. Whole-eye
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Cell culture technology has spread prolifically within a century, a variety of culture media has been designed. This review goes through the history, characteristics and current issues of animal-cell culture media. A literature search was performed on PubMed and Google
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MEM (Minimum Essential Medium Eagle)

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

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