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Merck

M5818

Sigma-Aldrich

Monoclonal Anti-hMps1 antibody produced in mouse

~2 mg/mL, clone N1, purified immunoglobulin, buffered aqueous solution

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About This Item

Número MDL:
Código UNSPSC:
12352203
NACRES:
NA.44

origen biológico

mouse

Nivel de calidad

conjugado

unconjugated

forma del anticuerpo

purified immunoglobulin

tipo de anticuerpo

primary antibodies

clon

N1, monoclonal

formulario

buffered aqueous solution

reactividad de especies

human

no debe reaccionar con

mouse

concentración

~2 mg/mL

técnicas

immunohistochemistry (frozen sections): suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 0.5-1.0 μg/mL using HeLa total cell extract

isotipo

IgG1

Nº de acceso UniProt

temp. de almacenamiento

−20°C

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... TTK(7272)

Descripción general

Human Mps1 kinase (hMps1) is a cell-cycle controlled protein that functions maximally during the M phase. hMps1 is involved in several cellular processes such as spindle assembly checkpoint, cell cycle progression, centrosome duplication and mitotic arrest . Monoclonal Anti-hMps1 is specific for human hMps1 and does not recognize mouse hMps1 (approx. 97 kDa).

Inmunógeno

recombinant catalytically inactive hMps1 (D663A) protein.

Aplicación

Monoclonal Anti-hMps1 is suitable for use in ELISA, microarray, immunoprecipitation, immunocytochemistry and immunohistochemistry (frozen sections). The product may also be used for western blot (0.5-1.0 μg/mL using HeLa total cell extract).

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Song-Tao Liu et al.
Molecular biology of the cell, 14(4), 1638-1651 (2003-04-11)
We have determined that the previously identified dual-specificity protein kinase TTK is the human orthologue of the yeast MPS1 kinase. Yeast MPS1 (monopolar spindle) is required for spindle pole duplication and the spindle checkpoint. Consistent with the recently identified vertebrate
Harold A Fisk et al.
Proceedings of the National Academy of Sciences of the United States of America, 100(25), 14875-14880 (2003-12-06)
The mitotic spindle is essential for the maintenance of genetic stability, and in budding yeast its assembly and function depend on the Mps1 protein kinase. Mps1p is required for centrosome duplication and the spindle checkpoint. Several recent reports demonstrate that
Volker M Stucke et al.
The EMBO journal, 21(7), 1723-1732 (2002-04-03)
Budding yeast Mps1p kinase has been implicated in both the duplication of microtubule-organizing centers and the spindle assembly checkpoint. Here we show that hMps1, the human homolog of yeast Mps1p, is a cell cycle-regulated kinase with maximal activity during M

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