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M5310

Sigma-Aldrich

Aceite de inmersión

light oil, suitable for mouse embryo cell culture, BioXtra

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About This Item

Número de CAS:
8042-47-5
Número CE:
232-455-8
Número MDL:
MFCD00131611
Código UNSPSC:
12352207
NACRES:
NA.75

esterilidad

sterile-filtered

Nivel de calidad

300

Línea del producto

BioXtra

formulario

light oil

condiciones de almacenamiento

protect from light

técnicas

cell culture | embryo: suitable

índice de refracción

n20/D 1.467 (lit.)

densidad

0.82-0.88 g/mL at 25 °C

formato

neat

Clave InChI

AEOVEGJBKQQFOP-DDVLFWKVSA-L

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Descripción general

Mineral oil or liquid petroleum is used for conservation of various types of cutltures. The advantages of using mineral oil are that the growth rate is decreased and the evaporation of water from the medium is almost completely eliminated. Thus, sub-culturing is reduced leading to increase in the viability of the culture. The first time mineral oil was used was in 1914, and it was used to overlay bacterial culture.

Aplicación

Mineral oil has been commonly used and recommended for overlaying mouse embryo cultures to prevent drying during the procedure.
Mineral oil is used for the following applications:
  • Used for cell viability studies in preparing the culture media or media supplements
  • Used tp protect array from evaporation by adding mineral oil around the array
  • Used to prepare the needle for microinjection (microinjection needle pulling, sharpening, and loading)
  • Used in PCR setup

Características y beneficios

Ready-to-use sterile filtered product conveniently packaged in PET bottles.

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

No data available

Punto de inflamabilidad (°C)

No data available

Equipo de protección personal

Eyeshields, Gloves

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Slobodan Beronja et al.
Methods in molecular biology (Clifton, N.J.), 961, 351-361 (2013-01-18)
We have recently developed a method for RNAi-mediated gene function analysis in skin (Beronja et al., Nat Med 16:821-827, 2010). It employs ultrasound-guided in utero microinjections of lentivirus into the amniotic cavity of embryonic day 9 mice, which result in
Mineral Oil Overlay for Conservation of Plant Tissue Cultures
Caplin SM
American Journal of Botany, 46(5), 324-329 (1959)
Hanyoup Kim et al.
Lab on a chip, 9(9), 1230-1235 (2009-04-17)
We report high-speed real-time PCR performed on an unmodified disposable polystyrene Petri dish. The reaction cycle relies solely on an infrared laser for heating; no conventional heater is required. Nanoliter droplets of PCR mixture as water-in-oil emulsions printed in an
Bei Zhao et al.
Scientific reports, 7(1), 11319-11319 (2017-09-14)
A microfluidic chip with a controllable and integrated piezoelectric pump was proposed and demonstrated, where the pump was designed as a micro-actuator based on polyvinylidene fluoride (PVDF) organic piezoelectric film. In this case, the pump should integrate with the microfluidics
Hanyoup Kim et al.
Optics express, 17(1), 218-227 (2009-01-09)
We report the successful application of low-power (approximately 30 mW) laser radiation as an optical heating source for high-speed real-time polymerase chain reaction (PCR) amplification of DNA in nanoliter droplets dispersed in an oil phase. Light provides the heating, temperature
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Protocolos

Determination of Water Content in Mineral oil Using Karl Fischer Titration

Summary application report for analysis of moisture in Mineral oil

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