M3567

Anti-Myosin Iβ (Nuclear) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Sinónimos: Anti-Myh 2, Anti-Myo1c, Anti-Myr 2
• Número MDL: MFCD05665241
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• conjugado: unconjugated
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• formulario: buffered aqueous solution
• mol peso: antigen ~120 kDa
• reactividad de especies: human, mouse, canine, rat
• técnicas: indirect immunofluorescence: 1:50 using mouse NIH3T3 cells; microarray: suitable; western blot: 1:1,000 using extract of human HeLa cells nuclei and dog MDCK cells
• Nº de acceso UniProt: O00159
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... MYO1C(4641) ; mouse ... Myo1c(17913) ; rat ... Myo1c(65261)

Descripción

Descripción general

Myosins belong to a superfamily of actin-based motor proteins comprising to date at least 15 classes. Myosin I (also designated myosin-1c, myo1c, M1C, myr 2 and myh 2) is a widely distributed single-headed myosin composed of an N-terminal motor domain, a calmodulin-light chain binding neck region, and a short C-terminal domain. In mammalian cells, myosin I is usually found in the cytoplasm and is especially enriched in perinuclear regions and dynamic cell margins. Myosin Iβ is found in a variety of cells such as kidney tubular cells and inner-ear hair cells.

Especificidad

Recognizes the heavy chain of the nuclear form of human myosin Iβ. Additional bands may be detected in some preparations.

Inmunógeno

synthetic peptide corresponding to amino acid residues 1-16 of the heavy chain of mouse myosin Iβ (nuclear form). This sequence is missing from the other myosin I isoforms as well as from other currently known myosins.

Aplicación

Anti-Myosin Iβ (Nuclear) antibody produced in rabbit has been used in:

• western blot
• confocal immunofluorescence
• immunogold labeling
• immunofluorescence
• co-immunoprecipitation experiments
• immunostaining

Acciones bioquímicas o fisiológicas

Myosin I is involved in cell motility, microvilli anchorage, vesicular and organelle transport and signal transduction. Myosin I associate with lipid membranes through its tail domain while its motor domain interacts with actin filaments. Myosin Iβ (nuclear) co-localizes with RNA polymerases I and II and possibly acts as a molecular motor to power transcription.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable