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M0696

Sigma-Aldrich

Anti-MNK2 (N-terminal) antibody produced in rabbit

enhanced validation

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Sinónimos:

Anti-GPRK7, Anti-MAP kinase interacting serine/threonine kinase 2, Anti-MKNK2

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.44

origen biológico

rabbit

Nivel de calidad

200

conjugado

unconjugated

forma del anticuerpo

affinity isolated antibody

tipo de anticuerpo

primary antibodies

clon

polyclonal

formulario

buffered aqueous solution

mol peso

antigen ~50 kDa

reactividad de especies

rat (predicted), human, mouse (predicted)

validación mejorada

recombinant expression
Learn more about Antibody Enhanced Validation

concentración

~1.0 mg/mL

técnicas

immunocytochemistry: 2-5 μg/mL using paraformadehyde fixed HEK-293T cells transfected with human MNK2
immunoprecipitation (IP): 2-4 μg using lysates of HEK-293T cells transfected with human MNK2
indirect immunofluorescence: suitable
western blot: 1-2 μg/mL using lysates of HEK-293T cells transfected with human MNK2

Nº de acceso UniProt

Q9HBH9

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... MKNK2(2872)
mouse ... Mknk2(17347)
rat ... Mknk2(299618)

Descripción general

MNK2 is a serine threonine kinase that phosphorylates endogenous eIF4E and thereby regulates protein translation and cell growth. This kinase inhibits apoptotic responses induced by arsenic trioxide. MNK2 has two distinct splice forms, namely; MNK2a and MNK2b. The localization and function of MNK2 splice variants are directed by their N and C termini . Anti-MNK2 (N-terminal) antibody is specific for human MNK2. The antibody is also expected to react with mouse and rat MNK2. In immunoblotting, staining of the MNK2 band is specifically inhibited by the immunizing peptide.

Inmunógeno

synthetic peptide corresponding to amino acids 51-64 of human MNK2 (corresponding to isoforms 1 and 2), conjugated to KLH via a C-terminal added cysteine residue. The corresponding sequence is conserved in human, rat, and mouse.

Aplicación

Anti-MNK2 (N-terminal) antibody is suitable for use in immunocytochemistry (2-5 μg/mL using paraformaldehyde fixed, MNK2-transfected, HEK-293T cells) and indirect immunofluorescence. The antibody may also be used for immunoprecipitation (2-4 μg) and western blot (1-2 μg/mL) using lysates of HEK-293T cells transfected with human MNK2.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

10 - Combustible liquids

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Y Shi et al.
Oncogene, 32(2), 190-197 (2012-03-01)
When mTOR inhibitor rapalogs prevent cap-dependent translation of cell-cycle proteins like c-myc, continuing tumor cell growth depends on cap-independent translation, which is mediated by internal ribosome entry sites (IRESes) located in the 5'-UTR (untranslated region) of transcripts. To investigate if
Senthilmurugan Ramalingam et al.
Cancers, 11(3) (2019-03-06)
Currently, there are no effective therapies for patients with triple-negative breast cancer (TNBC), an aggressive and highly metastatic disease. Activation of eukaryotic initiation factor 4E (eIF4E) by mitogen-activated protein kinase (MAPK)-interacting kinases 1 and 2 (Mnk1/2) play a critical role
Gert C Scheper et al.
Molecular and cellular biology, 23(16), 5692-5705 (2003-08-05)
The cap-binding eukaryotic initiation factor eIF4E is phosphorylated by the mitogen-activated protein (MAP) kinase-interacting kinases (Mnk's). Three forms of the Mnk's exist in human cells: Mnk1, Mnk2a, and Mnk2b. These last two are derived from the same gene by alternative
G C Scheper et al.
Molecular and cellular biology, 21(3), 743-754 (2001-01-12)
The cap-binding translation initiation factor eukaryotic initiation factor 4E (eIF4E) is phosphorylated in vivo at Ser209 in response to a variety of stimuli. In this paper, we show that the mitogen-activated protein kinase (MAPK) signal-integrating kinase Mnk2 phosphorylates eIF4E at
U Knauf et al.
Molecular and cellular biology, 21(16), 5500-5511 (2001-07-21)
Eukaryotic initiation factor 4E (eIF4E) is a key component of the translational machinery and an important modulator of cell growth and proliferation. The activity of eIF4E is thought to be regulated by interaction with inhibitory binding proteins (4E-BPs) and phosphorylation
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