M0446
Minimum Essential Medium Eagle, with Earl's salts
StableCell™, with Earle′s salts, stable glutamine, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Sinónimos:
Medio esencial mínimo Eagle, MEM
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About This Item
Código UNSPSC:
12352207
NACRES:
NA.75
Productos recomendados
Nombre del producto
Minimum Essential Medium Eagle, With Earle′s salts, stable glutamine, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Nivel de calidad
esterilidad
sterile-filtered
Formulario
liquid
técnicas
cell culture | mammalian: suitable
impurezas
endotoxin, tested
componentes
sodium pyruvate: no
HEPES: no
Hanks’ salts (2% CO2): no
NaHCO3: yes
Earle’s salts (5% CO2): no
stable glutamine: yes
phenol red: yes
L-glutamine: no
glucose: yes
Condiciones de envío
ambient
temp. de almacenamiento
2-8°C
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Descripción general
Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed they had specific nutritional requirements that could not be met by Eagle′s Basal Medium (BME). Subsequent studies using these and other cells in culture indicated additions to BME could be made to aid growth of a wider variety of fastidious cells.
MEM, which incorporates these modifications, includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has broadened the usefulness of this medium.
MEM, which incorporates these modifications, includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has broadened the usefulness of this medium.
Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.
Aplicación
Minimum Essential Medium Eagle has been used in murine fibroblast culture for in vitro cytocompatibility testing.
Otras notas
This product lacks L-Ala; L-Asn; L-Glu; Gly; L-Pro; L-Ser and L-Gln. It is supplemented with L-Ala-L-Gln dipeptide. This provides a more stable form of glutamine for cell culture. Free amino acid L-glutamine is known to be unstable in cell culture.
Información legal
StableCell is a trademark of Sigma-Aldrich Co. LLC
Opcional
también adquirido normalmente con este producto
Referencia del producto
Descripción
Precios
Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves
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