L7914
Lipoprotein, low density from human plasma
≥95% (SDS-PAGE), solution
Sinónimos:
β-Lipoprotein, LDL, Low density lipoprotein
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About This Item
Productos recomendados
origen biológico
human plasma
Nivel de calidad
Ensayo
≥95% (SDS-PAGE)
Formulario
solution
grupo funcional
ester
phospholipid
Condiciones de envío
wet ice
temp. de almacenamiento
2-8°C
Información sobre el gen
human ... APOB(338) , APOC2(344) , APOE(348)
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Aplicación
Lipoprotein, low density from human plasma has been used:
- as an additive in cholesterol-free RPMI media
- as a component in buffer to perform an assay for measuring lipid peroxidation
- to stimulate hepatic macrophages
- as a plasma protein to determine pazopanib unbound fraction (fu%) by equilibrium dialysis
Acciones bioquímicas o fisiológicas
LDL and HDL transport both dietary and endogenous cholesterol in the plasma. LDL is the main transporter of cholesterol and cholesteryl esters and makes up more than half of the total lipoprotein in plasma. LDL is absorbed by the liver and other tissues via receptor mediated endocytosis. The cytoplasmic domain of the LDL receptor facilitates the formation of coated pits; receptor-rich regions of the membrane. The ligand binding domain of the receptor recognizes apo-B100 on LDL, resulting in the formation of a clathrin-coated vesicle. ATP-dependent proton pumps lower the pH inside the vesicle resulting dissociation of LDL from its receptor. After loss of the clathrin coat the vesicles fuse with lysozomes, resulting in peptide and cholesteryl ester enzymatic hydrolysis. The LDL receptor can be recycled to the cell membrane. Insulin, tri-iodothyronine and dexamethasome have shown to be involved with the regulation of LDL receptor mediated uptake.
Precaución
Freezing of lipoprotein solutions may cause structural or compositional changes.
Propiedades físicas
Low density lipoproteins are smaller than VLDL and IDL (26 nm) (MW approximately 3.5 million) and more dense (~1.04). The protein component of LDL is apolipoprotein B100. LDL contains 20-22% protein, 10-15% triglycerides, 20-28% phospholipids, 37-48% cholesteryl esters and 8-10% cholesterol.
Forma física
Solution in 150 mM NaCl and 0.01% EDTA, pH 7.4
Otras notas
View more information on lipoprotein function and lipid transport at www.sigma-aldrich.com/enzymeexplorer
Cláusula de descargo de responsabilidad
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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Isolation and analysis of human plasma lipoproteins accumulating postrandial in an intermediate density fraction (d 1.006--1.019 g-ml).
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Artículos
Since cholesterol is a water-insoluble molecule it must be packaged for transport within the plasma. The particles that package cholesterol, cholesteryl esters, and triglycerides for transport, are called lipoproteins.
The potential for the prevention and treatment of cardiovascular disease through increased dietary intake of omega-3 (w-3) fish oils is not a recent scientific discovery.
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