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L1394

Sigma-Aldrich

Lectin from Triticum vulgaris (wheat)

agarose conjugate, saline suspension

Sinónimos:

WGA, Wheat germ agglutinin

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About This Item

Código UNSPSC:
12352202
NACRES:
NA.32

conjugado

agarose conjugate

Nivel de calidad

200

formulario

saline suspension

Extensión del etiquetado

~6 mg per mL

matriz

4% agarose

activación de la matriz

cyanogen bromide

espaciador de matriz

1 atom

tamaño de partícula

45-165 μm

capacidad

≥0.5 mg/mL binding capacity (ovomucoid)

temp. de almacenamiento

2-8°C

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Aplicación

Used for the purification of glycoproteins containing (D-N-acetylglucosamine)2 or N-acetylneuraminic acid.

Acciones bioquímicas o fisiológicas

WGA is not blood group specific but has an affinity for N-acetyl-β-D-glucosaminyl residues and N-acetyl-β-D-glucosamine oligomers. WGA contains no protein-bound carbohydrate.

Forma física

Suspension in 0.9% NaCl containing 15 mM Sodium azide.

Nota de preparación

Conjugates are prepared from affinity purified lectin.

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 3

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Bo F Hansen et al.
PloS one, 7(5), e34274-e34274 (2012-05-17)
There is controversy with respect to molecular characteristics of insulin analogues. We report a series of experiments forming a comprehensive characterisation of the long acting insulin analogues, glargine and detemir, in comparison with human insulin, IGF-1, and the super-mitogenic insulin
Liheng Yang et al.
The Journal of biological chemistry, 291(8), 3905-3917 (2015-12-20)
The normal cellular prion protein (PrP) is a glycosylphosphatidylinositol (GPI)-anchored cell surface glycoprotein. However, in pancreatic ductal adenocarcinoma cell lines, such as BxPC-3, PrP exists as a pro-PrP retaining its glycosylphosphatidylinositol (GPI) peptide signaling sequence. Here, we report the identification
D L Christie et al.
The Biochemical journal, 270(1), 57-61 (1990-08-15)
Chromaffin-granule membranes were separated into insoluble and soluble fractions after extraction with lithium di-iodosalicylate (LDIS). These fractions were characterized by one- and two-dimensional gel electrophoresis, and glycoproteins were detected after electroblotting with peroxidase-labelled concanavalin A and wheat-germ agglutinin (WGA). The
María-Ángeles Cortés-Gómez et al.
International journal of molecular sciences, 24(2) (2023-01-22)
In Alzheimer's disease (AD), the reduction in acetylcholinesterase (AChE) enzymatic activity is not paralleled with changes in its protein levels, suggesting the presence of a considerable enzymatically inactive pool in the brain. In the present study, we validated previous findings
Cynthia Tang et al.
Communications biology, 4(1), 161-161 (2021-02-07)
Mutations in CLN3 lead to photoreceptor cell loss in CLN3 disease, a lysosomal storage disorder characterized by childhood-onset vision loss, neurological impairment, and premature death. However, how CLN3 mutations cause photoreceptor cell death is not known. Here, we show that

Protocolos

Performing a Purification of Glycoproteins and Polysaccharides with Agarose Wheat Germ Lectin

This page shows how to purify glycoproteins and polysaccharides by affinity chromatography using Agarose Wheat Germ Lectin from GE Healthcare.

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