K0640

Anti-Potassium Channel K_V11.1 (HERG) Extracellular-FITC antibody produced in rabbit

affinity isolated antibody, lyophilized powder

• Sinónimos: Anti-KCNH2, Anti-Voltage gated K⁺ channel subfamily H member 2, Anti-ether-a-go-go-related channel 1
• Número MDL: MFCD02097212
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 200
• conjugado: FITC conjugate
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• formulario: lyophilized powder
• reactividad de especies: human
• técnicas: flow cytometry: 2-10 μg/mL using 1x10⁶ human live cells; immunocytochemistry: 1:25 using intact live HEK-KV11.1 transfected cells
• Nº de acceso UniProt: Q12809
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... KCNH2(3757)

Descripción

Descripción general

Anti-Potassium Channel K_V11.1 (HERG)- Extracellular-FITC (Voltage gated K⁺ channel subfamily H member 2, KCNH2, ether-a-go-go-related channel 1) is produced in rabbit using as immunogen the peptide AFLLKETEEGPPATEC corresponding to residues 430-445 of human K_V11.1 (HERG). Anti-K_V11.1 (HERG) antibody is directed against an extracellular epitope located between the S1 and S2 domains. The antibody is affinity purified on immobilized antigen and labeled with fluorescein isothiocyanate (FITC).

The gene Kv11.1, also referred to as KCNH2 (potassium voltage-gated channel subfamily H member 2), is mapped to human chromosome 7. The encoded voltage-activated potassium channel is a member of the ether-a-go-go (EAG) family.

Inmunógeno

peptide AFLLKETEEGPPATEC corresponding to residues 430-445 of human K_V11.1 (HERG).

Aplicación

Flow cytometry: a recommended working concentration of 2-10 μg per 1x10⁶cells was determined using intact live human cells.

Acciones bioquímicas o fisiológicas

The gene Kv11.1, also referred to as HERG (human ether-a-go-go related gene), encodes the α-subunit of the rapid component of the delayed rectifier potassium channel. The third repolarization of the action potential of human cardiomyocytes is carried out by this protein. Mutations in this gene have been associated with type II hereditary long QT syndrome (LQT2). This disease is characterized by prolongation of the QT interval, abnormal T wave, torsade de pointes, syncope and sudden death caused by cardiac arrest.

Forma física

Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA and 0.05% sodium azide.

Reconstitución

Reconstitute the lyophilized vial with 50 μL or 200 μL deionized water, depending on package size. Further dilutions should be made using a carrier protein such as BSA (1-3%).

Otras notas

Lyophilized powder can be stored intact at room temperature for several weeks. For extended storage, it should be stored at -20 °C or below. The reconstituted solution can be stored at 2-8 °C for up to 2 weeks. For longer storage, freeze in working aliquots. Avoid repeated freezing and thawing. Storage in “frost-free” freezers is not recommended. Centrifuge before use. Working dilution samples should be discarded if not used within 12 hours. The antibody is stable for at least 12 months when stored appropriately.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable