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Merck

I8015

Sigma-Aldrich

IgG from rat serum

technical grade, ≥80% (SDS-PAGE), buffered aqueous solution

Sinónimos:

Rat IgG

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About This Item

Número MDL:
Código UNSPSC:
12352203
NACRES:
NA.46

conjugado

unconjugated

Nivel de calidad

grado

technical grade

Análisis

≥80% (SDS-PAGE)

formulario

buffered aqueous solution

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

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Descripción general

IgG (immunoglobulin G) antibody is enriched in mother′s milk. IgG is a major class of immunoglobulin. It contributes to 10−20% of plasma protein and is regarded as one of the most abundant serum protein. It consists of four subclasses: IgG1, IgG2, IgG3 and IgG4. The IgG structure possesses four polypeptide chains containing two identical γ heavy (H) chains and two identical κ or λ light (L) chains of 50kDa and 25kDa respectively. The chains are interlinked with a disulfide bond. Both the L and the H chains consist of a variable domain at the N-terminal. The C-terminal bears′ constant domains: three in H chain with a hinge region and one in the L chain. The variable region of IgG antibody is specific to antigens and is highly conserved.

Aplicación

IgG from rat serum has been used:
  • To block programmed death ligand 1.
  • As a control antibody to study the role of integrin b1 in spindle morphology
  • In FACS (flow cytometry) staining
  • ELISA (enzyme linked immunosorbent assay)

IgG from rat serum was used to block non-specific staining during FACS of peritoneal cells isolated from BALB/c mice. It was used as positive control in VDR staining of male and female breast tumor cells. IgG from rat serum was injected in control mice in tumor transplantation studies.

Acciones bioquímicas o fisiológicas

IgG antibody has its function similar to IgM antibody in complement system activation. IgG participates in hypersensitivity type II and type III. It helps in opsonization, complement fixation and antibody-dependent cell-mediated cytotoxicity.
IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.2, containing 15 mM sodium azide

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

nwg

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Visite la Librería de documentos

The Laboratory Rat (2005)
Designing Antibodies, 216-216 (2012)
Awtar Krishan et al.
Cytometry. Part B, Clinical cytometry, 58(1), 53-60 (2004-03-03)
The present study was undertaken for quantitation of androgen (AR) and vitamin D (VDR) receptor expression in human male and female breast tumors by flow cytometry. Nuclei isolated from sections of paraffin-embedded tumors by pepsin digestion were treated for antigen
Proteasome inhibition with bortezomib depletes plasma cells and autoantibodies in experimental autoimmune myasthenia gravis
Gomez AM, et al.
Journal of Immunology, 1002539-1002539 (2011)
A Coronin 1--Dependent Decision Switch in Juvenile Mice Determines the Population of the Peripheral Naive T Cell Compartment
Lang MJ, et al.
Journal of Immunology, ji1700438-ji1700438 (2017)

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