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I7633

Sigma-Aldrich

IMDM

With ʟ-glutamine and HEPES, without sodium bicarbonate, powder, suitable for cell culture

Sinónimos:

Iscove′s MDM, Iscoves MDM, Iscove’s Modified Dulbecco’s Medium, IMDM

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About This Item

Número MDL:
MFCD00217469
Código UNSPSC:
12352207
NACRES:
NA.75

product name

Iscove′s Modified Dulbecco′s Medium, With L-glutamine and 25 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture

formulario

powder

técnicas

cell culture | hybridoma: suitable
cell culture | mammalian: suitable

componentes

phenol red: yes
glucose: yes
L-glutamine: yes

Condiciones de envío

ambient

temp. de almacenamiento

2-8°C

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Descripción general

Iscove′s Modified Dulbecco′s medium is a modification of Dulbecco′s Modified Eagle′s Medium (DME) containing selenium, additional amino acids, and vitamins, sodium pyruvate, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer, and potassium nitrate instead of ferric nitrate. Iscove′s medium supports murine B lymphocytes, hemopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells.

Aplicación

Iscove′s Modified Dulbecco′s Medium has been used to culture KG1a cells (human leukemic cell line) and Chinese hamster ovary (CHO) DG44 cell line.
Reported to support murine B lymphocytes, hematopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells.

Cantidad

Formulated to contain 17.7 grams of powder per liter of medium.

Reconstitución

Supplement with 3.024 g/L sodium bicarbonate.

suplemento

Referencia del producto
Descripción
Precios

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Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Protocolos

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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