HPA010136
Anti-POR antibody produced in rabbit
Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Sinónimos:
Anti-CPR antibody produced in rabbit, Anti-NADPH--cytochrome P450 reductase antibody produced in rabbit, Anti-P450R antibody produced in rabbit
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About This Item
conjugado:
unconjugated
application:
IF
IHC
IHC
clon:
polyclonal
reactividad de especies:
human, rat, mouse
citations:
6
técnicas:
immunoblotting: 0.04-0.4 μg/mL
immunofluorescence: 0.25-2 μg/mL
immunohistochemistry: 1:200-1:500
immunofluorescence: 0.25-2 μg/mL
immunohistochemistry: 1:200-1:500
Productos recomendados
origen biológico
rabbit
Nivel de calidad
conjugado
unconjugated
forma del anticuerpo
affinity isolated antibody
tipo de anticuerpo
primary antibodies
clon
polyclonal
Línea del producto
Prestige Antibodies® Powered by Atlas Antibodies
Formulario
buffered aqueous glycerol solution
reactividad de especies
human, rat, mouse
validación mejorada
orthogonal RNAseq
Learn more about Antibody Enhanced Validation
técnicas
immunoblotting: 0.04-0.4 μg/mL
immunofluorescence: 0.25-2 μg/mL
immunohistochemistry: 1:200-1:500
secuencia del inmunógeno
KEEVPEFTKIQTLTSSVRESSFVEKMKKTGRNIIVFYGSQTGTAEEFANRLSKDAHRYGMRGMSADPEEYDLADLSSLPEIDNALVVFCMATYGEGDPTDNAQDFYDWLQETDVDLSGVKFAVFGLGNKTYEHFNA
Nº de acceso UniProt
Condiciones de envío
wet ice
temp. de almacenamiento
−20°C
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... POR(5447)
Descripción general
Cytochrome p450 oxidoreductase (POR) is a 78 kDa membrane-bound diflavin oxidoreductase, which is located on the cytoplasmic side of the endoplasmic reticulum and the outer membrane of the nuclear envelope. The gene is located on human chromosome 7q11.23. POR consists of flavin mononucleotide (FMN) binding domain and nicotinamide adenine dinucleotide phosphate (NADPH)/flavin adenine dinucleotide (FAD) binding domain.
POR (P450 oxidoreductase) is an 80kDa membrane-bound enzyme, localized to endoplasmic reticulum (ER). It is a key enzyme in the P450-mon-oxygenase drug-metabolizing system. It belongs to the diflavin reductases.
Inmunógeno
NADPH--cytochrome P450 reductase recombinant protein epitope signature tag (PrEST)
Aplicación
All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry.
The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies® protocols and other useful information.
The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies® protocols and other useful information.
Anti-POR antibody produced in rabbit has been used in elucidating the mechanisms responsible for the altered hepatic drug-metabolizing activity in acute lung injury (ALI) rats.
Acciones bioquímicas o fisiológicas
Cytochrome p450 oxidoreductase (POR) binds to two cofactors, FAD and FMN, which catalyses the transfer of reducing equivalents from NADPH to various physiological acceptors, such as cytochrome b5, squalene monooxygenase and heme oxygenase. It facilitates electron transfer from β-NADPH to cytochrome P450 (CYP) in drug metabolism. Mutations in this gene is associated with congenital adrenal hyperplasia, Antley-Bixler syndrome (ABS), P450C17 and P450C21 deficiency, amenorrhea and polycystic ovaries.
POR (P450 oxidoreductase) functions as an electron donor for all microsomal CYP (cytochrome P450) enzymes. It plays an essential role in RA (retinoic acid) metabolism, as POR insufficient mice have markedly increased levels of RA. In human acute myeloid leukemia cells, the expression of POR is controlled by all-trans retinoic acid (ATRA) and by 1,25-dihydroxyvitamin D3. Studies in human cancer cell lines show that the activity of hypoxia-activated prodrugs (HAP) is dependent on POR enzyme.
Características y beneficios
Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.
Every Prestige Antibody is tested in the following ways:
Every Prestige Antibody is tested in the following ways:
- IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
- Protein array of 364 human recombinant protein fragments.
Ligadura / enlace
Corresponding Antigen APREST71930
Forma física
Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide
Información legal
Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 1
Equipo de protección personal
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Certificados de análisis (COA)
Lot/Batch Number
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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Role of active site loop in coenzyme binding and flavin reduction in cytochrome P450 reductase
Mothersole RG, et al.
Archives of Biochemistry and Biophysics, 606(6), 111-119 (2016)
Altered hepatic drug-metabolizing activity in rats suffering from hypoxemia with experimentally induced acute lung impairment
Hori Y, et al.
Xenobiotica, 48(6), 576-583 (2018)
Elżbieta Gocek et al.
PloS one, 9(3), e91752-e91752 (2014-03-20)
Acute myeloid leukemia (AML) cell lines can be driven to differentiate to monocyte-like cells by 1,25- dihydroxyvitamin D3 (1,25D) and to granulocyte-like cells by all-trans-retinoic acid (ATRA). Both compounds activate their specific intracellular receptors, vitamin D receptor (VDR) and retinoic
Genes for two autosomal recessive forms of chronic granulomatous disease assigned to 1q25 (NCF2) and 7q11. 23 (NCF1).
Francke U, et al.
American Journal of Human Genetics, 47(3), 483-483 (1990)
Wei-Cheng Huang et al.
Structure (London, England : 1993), 21(9), 1581-1589 (2013-08-06)
NADPH-cytochrome P450 reductase is a key component of the P450 mono-oxygenase drug-metabolizing system. There is evidence for a conformational equilibrium involving large-scale domain motions in this enzyme. We now show, using small-angle X-ray scattering (SAXS) and small-angle neutron scattering, that
Artículos
Phase I biotransformation reactions introduce or expose functional groups on the drug with the goal of increasing the polarity of the compound. Although Phase I drug metabolism occurs in most tissues, the primary and first pass site of metabolism occurs during hepatic circulation.
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