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Merck

H9537

Sigma-Aldrich

Anti-HMG-1 antibody, Mouse monoclonal

clone HAP46.5, purified from hybridoma cell culture

Sinónimos:

Anti-Amphoterin, Anti-HMGB1, Anti-High-Mobility-Group protein

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About This Item

Número MDL:
Código UNSPSC:
12352203
NACRES:
NA.41

origen biológico

mouse

Nivel de calidad

conjugado

unconjugated

forma del anticuerpo

purified immunoglobulin

tipo de anticuerpo

primary antibodies

clon

HAP46.5, monoclonal

Formulario

buffered aqueous solution

mol peso

antigen ~25 kDa

reactividad de especies

canine, mouse, rat, chicken, human

técnicas

indirect ELISA: suitable
microarray: suitable
western blot: 0.5-1 μg/mL using total cell extract of human MCF7 breast carcinoma cells

isotipo

IgG1

Nº de acceso UniProt

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... HMGB1(3146)
mouse ... Hmgb1(15289)
rat ... Hmgb1(25459)

Descripción general

High-mobility group-1 protein (HMG-1) or amphoterin is a nuclear DNA-binding protein that facilitates the bending and transcription of DNA. It also has a pivotal role in the regulation of chromatin structure, DNA replication, ligation and repair. Monoclonal anti-HMG-1 antibody can be used as a vital tool to study the biology of transcription. It can also be used in indirect ELISA and immunoblotting (25kD approx.). Mouse anti-HMG-1 antibody reacts specifically with HMG-1 of canine, mouse, chicken, rat and human.
Monoclonal Anti-HMG-1 (mouse IgG1 isotype) is derived from the HAP46.5 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mouse immunized with purified human recombinant HMG-1. HMG-1 (High-Mobility-Group protein, amphoterin) is a highly conserved protein with more than 95% amino acid identity between rodent and human HMG-1. It is a prevalent non-histone chromatin component and a non-sequence specific DNA binding protein. HMG-1 consists of two homologous HMG boxes rich in basic amino acids and an acidic tail at the carboxy-terminus.

Inmunógeno

purified recombinant human HMG-1.

Aplicación

Monoclonal Anti-HMG-1 antibody is suitable for use in immunochemical applications such as ELISA and immunoblotting (approx. 25 kDa).
Monoclonal anti-HMG-1 antibody can be used in immunofluorescent staining. It can also be used in microarray and western blotting.

Acciones bioquímicas o fisiológicas

High-Mobility-Group protein, amphoterin, HMG-1 is involved in the regulation of chromatin structure as well as being involved, either as positive or negative factors with various aspects of DNA replication, transcription, repair, and ligation. HMG-1, identified as a membrane associated protein termed “amphoterin,” mediates neurite outgrowth, tumor outgrowth, and metastasis. It participates in plasminogen activation and is recognized as a late mediator of endotoxin lethality in mice.

Idoneidad

Monoclonal Anti-HMG-1 recognizes human, canine, mouse, rat, and chicken HMG-1.

Forma física

Solution in 0.01 M phosphate buffered saline containing 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Opcional

Referencia del producto
Descripción
Precios

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

nwg

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Visite la Librería de documentos

The double life of HMGB1 chromatin protein: architectural factor and extracellular signal
Muller S, et al.
The Embo Journal, 20(16), 4337-4340 (2001)
Xiaoping Qing et al.
Immunology letters, 121(1), 61-73 (2008-09-30)
Although anti-DNA antibodies have been decisively linked to the pathogenesis of lupus nephritis, the mechanisms have not been conclusively determined. Recently, we reported that anti-DNA antibodies may contribute to kidney damage by upregulation of proinflammatory genes in mesangial cells (MC)
Non-histone nuclear factor HMGB1 is phosphorylated and secreted in colon cancers
Kang HJ, et al.
Laboratory Investigation; a Journal of Technical Methods and Pathology, 89(8), 948-948 (2009)
HMG1 protein stimulates DNA end joining by promoting association of DNA molecules via their ends
Muller S, et al.
European Journal of Biochemistry, 267(13), 4088-4097 (2000)
Jing Xie et al.
Molecular medicine reports, 13(6), 5335-5341 (2016-04-29)
High mobility group box 1 (HMGB1) protein serves an important role in myocardial ischemia/reperfusion (I/R) injury. MicroRNAs (miRNAs) are a group of small non‑coding RNAs that regulate numerous signaling pathways involved in myocardial I/R injury. The present study aimed to investigate whether

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