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H5000

Sigma-Aldrich

Hexokinase from Saccharomyces cerevisiae

Type III, lyophilized powder, ≥25 units/mg protein (biuret)

Sinónimos:

ATP:D-Hexose-6-phosphotransferase, Hexokinase from yeast

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About This Item

Número de CAS:
9001-51-8
Comisión internacional de enzimas:
2.7.1.1 (BRENDA, IUBMB)
Número CE:
232-611-5
Número MDL:
MFCD00131321
Código UNSPSC:
12352204
eCl@ss:
32160410

tpo

Type III

formulario

lyophilized powder

actividad específica

≥25 units/mg protein (biuret)

mol peso

dimer 110 kDa

Nº de acceso UniProt

P04806
P04807

actividad extraña

glucose-6-phosphate dehydrogenase and phosphoglucose isomerase ≤10%

temp. de almacenamiento

−20°C

Información sobre el gen

bakers yeast ... HXK1(850614) , HXK2(852639)

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Acciones bioquímicas o fisiológicas

Catalyzes the phosphorylation of D-hexose sugars at the C6 position utilizing ATP as a phosphate source.

The rate of phosphorylation varies with different hexoses (pH 7.5, 30 °C).
D-fructose KM: 0.33 mM
D-glucose KM: 0.12 mM
D-mannose KM: 0.05 mM

Yeast hexokinase exists as two similar isoforms, PI and PII (A and B), with isoelectric points of 5.25 and 4, respectively.

Molecular Weight: ~ 54 kDa (monomer)
~110 kDa (dimer)
Optimal pH: 7.5 to 9.0
Extinction Coefficient: E1% = 8.85 (PI) and 9.47 (PII) at 280 nm

Activators: Hexokinase requires Mg2+ ions (KM = 2.6 mM) for activity. Hexokinase is activated by catecholamines and related compounds.

Inhibitors: sorbose-1-phosphate, polyphosphates, 6-deoxy-6-fluoroglucose, 2-C-hydroxy-methylglucose, xylose, lyxose, and thiol reactive compounds (Hg2+ and 4-chloromercuribenzoate)

Definición de unidad

One unit will phosphorylate 1.0 μmole of D-glucose per min at pH 7.6 at 25 °C, unless otherwise indicated below.

Forma física

Lyophilized powder containing approx. 15% sodium citrate

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Charles Betz et al.
The Journal of cell biology, 203(4), 563-574 (2014-01-05)
Target of rapamycin (TOR) forms two conserved, structurally distinct kinase complexes termed TOR complex 1 (TORC1) and TORC2. Each complex phosphorylates a different set of substrates to regulate cell growth. In mammals, mTOR is stimulated by nutrients and growth factors
Markus Weiler et al.
Proceedings of the National Academy of Sciences of the United States of America, 111(1), 409-414 (2013-12-25)
A hypoxic microenvironment induces resistance to alkylating agents by activating targets in the mammalian target of rapamycin (mTOR) pathway. The molecular mechanisms involved in this mTOR-mediated hypoxia-induced chemoresistance, however, are unclear. Here we identify the mTOR target N-myc downstream regulated
François Vaillant et al.
Cancer cell, 24(1), 120-129 (2013-07-13)
The prosurvival protein BCL-2 is frequently overexpressed in estrogen receptor (ER)-positive breast cancer. We have generated ER-positive primary breast tumor xenografts that recapitulate the primary tumors and demonstrate that the BH3 mimetic ABT-737 markedly improves tumor response to the antiestrogen
Daniel Romaker et al.
Proceedings of the National Academy of Sciences of the United States of America, 111(17), 6335-6340 (2014-04-16)
MicroRNAs (miRNAs) are major posttranscriptional regulators of a wide variety of biological processes. However, redundancy among most miRNAs has made it difficult to identify their in vivo functions. We previously demonstrated that global inhibition of miRNA biogenesis in Xenopus resulted
Seok-Hyung Kim et al.
PLoS genetics, 9(6), e1003563-e1003563 (2013-06-21)
Multiple Acyl-CoA Dehydrogenase Deficiency (MADD) is a severe mitochondrial disorder featuring multi-organ dysfunction. Mutations in either the ETFA, ETFB, and ETFDH genes can cause MADD but very little is known about disease specific mechanisms due to a paucity of animal
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Protocolos

Enzymatic Assay of Hexokinase

Enzymatic Assay of Hexokinase

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