Saltar al contenido
Merck

H3284

Sigma-Aldrich

Anti-Histone Deacetylase 1 (HDAC1) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Sinónimos:

Anti-GON-10, Anti-HD1, Anti-KDAC1, Anti-RPD3, Anti-RPD3L1

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización


About This Item

Número MDL:
Código UNSPSC:
12352203
NACRES:
NA.41

origen biológico

rabbit

Nivel de calidad

conjugado

unconjugated

forma del anticuerpo

IgG fraction of antiserum

tipo de anticuerpo

primary antibodies

clon

polyclonal

formulario

buffered aqueous solution

mol peso

antigen 65 kDa

reactividad de especies

human, mouse

envase

antibody small pack of 25 μL

técnicas

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using human lymph node sections
immunoprecipitation (IP): 5-10 μL using whole lysate of NIH3T3 cells
microarray: suitable
western blot: 1:20,000 using nuclear extract from HeLa human epithelioid carcinoma cells

Nº de acceso UniProt

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... HDAC1(3065)
mouse ... Hdac1(433759)

Descripción general

Acetylation is a histone modification in which epsilon amino group of conserve lysine residue is modified with enzymes known as histone acetyltransferases (HATs). HATs are present in two forms nuclear and cytoplasmic. Histone deacetylases are competating enzymes of HATs and are often associated with transcriptional repression and nucleosome condensation. Anti-Histone Deacetylase 1 (HDAC1) antibody can be used in chromatin immunoprecipitation. . This antibody can also be used in double immunofloresence labeling. Anti-Histone Deacetylase 1 (HDAC1) antibody specifically recognizes histone deacetylase 1and reacts specifically with HDAC1 of human, rat and mouse.
Histone acetylation is a dynamic process whose levels are determined by the net activities of HATs and the competing enzymes histone deacetylases (HDACs). Both activities are associated with the nuclear matrix. Six or seven different mammalian HDACs have been described. HDAC1, HDAC2 and HDAC3 are similar to yeast Rpd3 protein, while HDAC4, HDAC5 and HDAC6 are similar to yeast Hda1 protein. Histone deacetylases activities were often, but not always, associated with transcriptional repression and nucleosomal condensations. HDAC1, HDAC2 and several other HDACs are the catalytic subunits of different multiprotein regulatory complexes.
Histone deacetylase 1 (HDAC1) is located in the nucleus. It belongs to class I of histone deacetylases. HDAC1 is located on human chromosome 1p35.

Especificidad

Anti-Histone Deacetylase 1 specifically recognizes histone deacetylase 1 by immunoblotting and immunoprecipitation (65 kDa). An additional band of lower molecular weight may be detected in some cell line extracts by immunoblotting. Staining of HDAC1 by immunoblotting is specifically inhibited with the immunizing peptide. The antibody is also useful for the detection of HDAC1 by immunohistochemistry. The epitope(s) recognized by the antibody is resistant to routine formalin-fixation and paraffin-embedding, unless there is a protease digestion. The antibody reacts with HDAC1 of human, rat and mouse origin.

Inmunógeno

synthetic peptide corresponding to amino acids 466-482 of human and mouse HDAC1.

Aplicación

Anti-Histone Deacetylase 1 (HDAC1) antibody can be used in immunoblotting; immunoprecipitation and immunohistochemistry. It is also useful in chromatin immunoprecipitation.
Anti-Histone Deacetylase 1 (HDAC1) antibody has been used in immunohistochemical staining and chromatin immunoprecipitation (ChIP) analyses.
Anti-Histone Deacetylase 1 is suitable for immunoblotting; immunoprecipitation and immunohistochemistry. (IHC)
Immunoblotting: a minimum working dilution of 1:20,000 is determined using a nuclear extract of HeLa human epithelioid carcinoma cell line.
Immunoblotting: a minimum working dilution of 1:2,000 is determined using a whole extract of PC-12 rat pheochromocytoma cell line.
Immunoprecipitation: a recommended working volume of 5-10 ml is determined using a whole lysate of NIH 3T3 cells.
Indirect immunoperoxidase staining: a minimum working dilution of 1:500 is determined using protease-digested, formalin-fixed, paraffin-embedded human lymph node sections.

Acciones bioquímicas o fisiológicas

Histone deacetylase 1 (HDAC1) prevents the infection of influenza A virus (IAV). The protein plays important roles in DNA repair, splicing, regulation of gene expression and cell division. Overexpression of this gene can be seen in cancers like breast, renal cell cancer, and classical Hodgkin′s lymphoma.

Forma física

Solution in 0.01 M phosphate bufffered saline, pH 7.4, containing 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

¿No encuentra el producto adecuado?  

Pruebe nuestro Herramienta de selección de productos.

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Detection of fetomaternal genotype associations in early-onset disorders: evaluation of different methods and their application to childhood leukemia
Healy J, et al.
BioMed Research International, 2010(31) (2010)
PDT-induced epigenetic changes in the mouse cerebral cortex: a protein microarray study
Demyanenko S V, et al.
Biochim. Biophys. Acta Gen. Subj., 1840(1), 262-270 (2014)
Influenza A virus dysregulates host histone deacetylase 1 that inhibits viral infection in lung epithelial cells
Nagesh P T and Husain M
Journal of virology, JVI-00126 (2016)
Direct p53 transcriptional repression: in vivo analysis of CCAAT-containing G2/M promoters
Imbriano C, et al.
Molecular and cellular biology, 25(9), 3737-3751 (2005)
Aymone Gurtner et al.
PloS one, 3(4), e2047-e2047 (2008-04-24)
The regulation of gene transcription requires posttranslational modifications of histones that, in concert with chromatin remodeling factors, shape the structure of chromatin. It is currently under intense investigation how this structure is modulated, in particular in the context of proliferation

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico