Saltar al contenido
Merck
Todas las fotos(1)

Key Documents

View All Documentation

H1506

Sigma-Aldrich

3α-Hydroxysteroid Dehydrogenase from Pseudomonas testosteroni

≥15 units/mg protein

Sinónimos:

3α-Hydroxysteroid:NAD(P)+ oxidoreductase

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Todas las fotos(1)

About This Item

Número de CAS:
9028-56-2
Comisión internacional de enzimas:
1.1.1.50 (BRENDA, IUBMB)
Número CE:
232-839-5
Número MDL:
MFCD00130298
Código UNSPSC:
12352204
NACRES:
NA.54

origen biológico

bacterial (Pseudomonas testosteroni)

formulario

lyophilized solid

actividad específica

≥15 units/mg protein

purificado por

chromatography

composición

Protein, ≥25%

actividad extraña

β-hydroxysteroid dehydrogenase ≤0.5%
alcohol dehydrogenase ≤0.05%

temp. de almacenamiento

−20°C

¿Está buscando productos similares? Visita Guía de comparación de productos

Aplicación

3α-Hydroxysteroid Dehydrogenase from Pseudomonas testosterone has been used:
  • in the preparation of composite electrode platform to for the determination of androsterone
  • in potassium phosphate buffer for the bioluminescent assay of bile acids in serum
  • for total bile salts assay using an enzymatic method

Acciones bioquímicas o fisiológicas

3α-hydroxysteroid dehydrogenase (3α-HSD) may help to modulate the occupancy of the androgen receptor (AR) in androgen target tissues. It may also act in hindering androgen action.

Definición de unidad

One unit will oxidize 1.0 μmole of androsterone per min at pH 8.9 at 25 °C in the presence of β-NAD+.

Forma física

Lyophilized powder containing potassium phosphate buffer salt, EDTA, and DL-dithiothreitol

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Bin Li et al.
Proteins, 71(2), 670-683 (2007-11-03)
Experimentally determined protein tertiary structures are rapidly accumulating in a database, partly due to the structural genomics projects. Included are proteins of unknown function, whose function has not been investigated by experiments and was not able to be predicted by
Expression and characterization of recombinant type 2 3alpha-hydroxysteroid dehydrogenase (HSD) from human prostate: demonstration of bifunctional 3alpha17beta-HSD activity and cellular distribution
Lin HK, et al.
Molecular Endocrinology, 11(13), 1971-1984 (1997)
Chiaki Hidai et al.
Bioprocess and biosystems engineering, 32(5), 569-573 (2008-11-20)
Developing methods that result in targeting of therapeutic molecules in gene therapies to target tissues has importance, as targeting can increase efficacy and decrease off target-side-effects. Work from my laboratory previously showed that the extracellular matrix protein Del1 is organized
Brandon Bartling et al.
The Analyst, 134(5), 973-979 (2009-04-22)
An electrochemical sensor, based on thick-film screen-printed Ir/C working and counter electrodes, was developed for the detection of total bile acid concentration in a physiological fluid for potential patient management in patients with liver disease. Current electrochemical methods of detecting
Frank Hoffmann et al.
Journal of biotechnology, 129(1), 131-139 (2007-01-30)
3alpha-Hydroxysteroid dehydrogenase/carbonyl reductase (3alpha-HSD/CR) from Comamonas testosteroni belongs to the short chain dehydrogenase/reductase (SDR) protein superfamily and catalyzes the oxidoreduction of a variety of steroid substrates, including the steroid antibiotic fusidic acid. The enzyme also mediates the carbonyl reduction of
Ver todos los artículos relacionados

Protocolos

Enzymatic Assay of 3α-Hydroxysteroid Dehydrogenase with Androsterone (EC 1.1.1.50)

Enzymatic Assay of 3α-Hydroxysteroid Dehydrogenase with Androsterone (EC 1.1.1.50)

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico