H1387
Hanks′ Balanced Salts
Modified, without phenol red and sodium bicarbonate, powder, suitable for cell culture
Sinónimos:
HBSS
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About This Item
Productos recomendados
Nivel de calidad
formulario
powder
técnicas
cell culture | mammalian: suitable
temp. de almacenamiento
2-8°C
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Descripción general
Balanced salt solution (BSS) serves as an irrigating, transporting, and diluting fluid while maintaining intra- and extracellular osmotic balance. It also provides cells with water and certain bulk inorganic ions essential for normal cell metabolism. BSS when combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism. In addition, it also provides a buffering system to maintain the medium within the physiological pH range (7.2-7.6).
Aplicación
Hanks′ Balanced Salts has been used:
- for diluting digested tissues during isolation of pancreatic blood vessels
- for washing mouse intestinal mucosal cells before lysis and sonication
- as an immersion solution to test the in vitro bioactivity of poly(ε-caprolactone)-coated hydroxyapatite scaffold derived from cuttlefish bone
Cantidad
Formulated to contain 9.7 grams of powder per liter of medium.
Reconstitución
Supplement with 0.35 g/L sodium bicarbonate.
Otras notas
The pH is adjusted after addition of sodium bicarbonate. Investigators who work with stem cells or cell clones frequently prefer media without phenol red.
suplemento
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, type N95 (US)
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Protocolos
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
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