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GE28-9852-56

rProtein A/Protein G GraviTrap™

Cytiva 28-9852-56, pack of 10 × 1 mL

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About This Item

Código UNSPSC:
23151816
NACRES:
NA.56

envase

pack of 10 × 1 mL

fabricante / nombre comercial

Cytiva 28-9852-56

condiciones de almacenamiento

(20% Ethanol)

lecho volumen

1 mL

capacidad

35 mg binding capacity (human IgG)(Protein binding capacity is protein-to-protein dependent.)

temp. de almacenamiento

2-8°C

Descripción general

rProtein A Protein G GraviTrap™ includes ten ready-to-use columns prepacked with 1 mL of a mixture of 50% rProtein A Sepharose™ Fast FLow and 50% protein G Sepharose™ 4 Fast Flow.

Aplicación

rProtein A Protein G GraviTrap™ are prepacked gravity-fLow columns designed for fast and efficient manual purification of monoclonal and polyclonal antibodies, antibody fragments from cell culture supernatant, and biological fluids. The antibodies are simply captured with high specificity on Protein A and Protein G ligands in gravity-fLow columns. You do not need any other instrument with this protocol because the entire process relies on the flow of gravity. The yield varies from 20 to 50 mg of purified antibodies depending on the ligand you use. The columns are reusable up to five times.

Características y beneficios

  • Prepacked gravity-fLow columns provide convenience, save time, and increase the reliability of your results.
  • Purification of antibodies in 30 min allows you to quickly obtain and store your target Protein in order to avoid the inherent dangers of Protein instability and degradation associated with sLow and long purification runs.
  • High purity and recovery with purity > 95% in one step and recovery typically 70% to 80%.
  • High binding capacity such as Protein A/Protein G GraviTrap™ ~ 35 mg human IgG/column.

Almacenamiento y estabilidad

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Nota de análisis

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Información legal

GraviTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva

Pictogramas

Flame
GHS02

Palabra de señalización

Warning

Frases de peligro

H226

Código de clase de almacenamiento

3 - Flammable liquids

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Certificados de análisis (COA)

Lot/Batch Number

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Artículos

Performing a Purification of IgG Antibodies with Protein G GraviTrap™

This page shows how to separate IgG antibodies by affinity chromatography using Protein G GraviTrap from GE Healthcare.

Purification Using Protein A-based Chromatography Media

This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

This page shows how to perform sample desalting and buffer exchange for affinity chromatography of antibodies.

Protocolos

Performing a Purification of IgG Antibodies with Protein G GraviTrap™

This page shows how to separate IgG antibodies by affinity chromatography using Protein G GraviTrap from GE Healthcare

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