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GE28-9355-98

Dextrin Sepharose™ High Performance

Cytiva 28-9355-98, pack of 100 mL

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About This Item

Código UNSPSC:
41106500
NACRES:
NA.56

ligand

dextrin

envase

pack of 100 mL

fabricante / nombre comercial

Cytiva 28-9355-98

condiciones de almacenamiento

(20% Ehtanol)

Matriz

highly cross-linked 6% agarose

tam. prom. pieza

34 μm

cleaning in place

2-13

Intervalo de trabajo

>7

capacidad

~7 mg binding capacity (MBP- ΔSal/ml medium Approx. 16 mg MBP- ßGal/ml medium)

temp. de almacenamiento

2-8°C

Categorías relacionadas

Descripción general

Binding capacity is protein dependent. Dynamic binding capacity was tested with MBP-?Sal (MBP2*-paramyosin-?Sal, Mr approx. 70 000, multimer in solution), and MBP-ßGal (MBP2*-beta-galactosidase, Mr approx. 158 000, multimer in solution).
Dextrin Sepharose™ High Perfromance is an affinity media for purification of proteins tagged with Maltose Binding protein (MBP).

Aplicación

Dextrin Sepharose™ High Performance and provide convenient affinity purification of recombinant Proteins tagged with maltose binding Protein (MBP). This robust and stable Sepharose™ High Performance based medium enables elution of target Protein in narrow peaks, which minimizes the need for further concentration steps.

Características y beneficios

  • Dextrin Sepharose™ High Performance is a robust, high resolution medium based on the 34 μm Sepharose™ High Performance matrix
  • The small, evenly sized beads ensure that MBP-tagged Proteins elute in narrow peaks, thus minimizing the need for further concentration steps
  • Physiological conditions and mild elution preserve target Protein activity
  • Dextrin Sepharose™ High Performance tolerates all commonly used aqueous buffers and is easily regenerated using 0.5 M NaOH allowing the same column to be used for repeated purifications

Almacenamiento y estabilidad

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Nota de análisis

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Información legal

Sepharose is a trademark of Cytiva

Pictogramas

Flame
GHS02

Palabra de señalización

Warning

Frases de peligro

H226

Código de clase de almacenamiento

3 - Flammable liquids

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Certificados de análisis (COA)

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Artículos

Characteristics of Dextrin Sepharose® High Performance Products

This page shows the characteristics of Dextrin Sepharose High Performance products from GE Healthcare.

Protocolos

Column Packing and Preparation

This page describes efficient column packing and preparation for affinity chromatography of tagged proteins using GE Healthcare products.

Column Cleaning for Ion Exchange Chromatography and Chromatofocusing

This page covers detailed information on cleaning procedures and recommended flow for column cleaning.

Desalting/Buffer Exchange and Concentration for Affinity Chromatography of Tagged Proteins

This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.

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