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GE28-9031-30

Streptavidin High Performance Spintrap™

Cytiva 28-9031-30, pack of 16 columns

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About This Item

Código UNSPSC:
41106500
NACRES:
NA.25

Características

wetted part: no

envase

pack of 16 columns

fabricante / nombre comercial

Cytiva 28-9031-30

condiciones de almacenamiento

(20% Ehtanol)
 (20% Ethanol)

lecho volumen

800 μL

Matriz

highly cross-linked 6% agarose

tam. prom. pieza

34 μm

cleaning

2-10.5

Intervalo de trabajo

4-9

capacidad

>300 nmol/mL binding capacity(biotin/ml medium 6 mg biotinylated BSA/ml medium)
 binding capacity(>300 nmol biotin/ml medium 6 mg biotinylated BSA/ml medium)

temp. de almacenamiento

2-8°C

Descripción general

Streptavidin HP SpinTrap™ are prepacked, single-use spin columns and 96-well filter plates for the preparation of protein samples and enrichment of proteins of interest from clarified cell lysates and biological fluids.

Aplicación

Streptavidin HP SpinTrap™ can be used for Protein enrichment, where a biotinylated antibody (or similar affinity molecule) is attached to the streptavidin and the Protein of interest is enriched through the affinity interaction with the antibody. The columns can also be used for the direct enrichment of Proteins that are biotinylated. By binding a specific biotinylated Protein to the column, Protein-Protein interactions can also be investigated.

Características y beneficios

  • For simple, small-scale Protein enrichment through immobilized biotinylated biomolecules.
  • Fast and flexible protocol with elution conditions formatted for both electrophoresis and LC-MS analysis workflows.
  • Useful for exploiting either the strong interaction of biotin and streptavidin or the somewhat weaker interaction of 2-iminobiotin and streptavidin.
  • Each column is packed with Streptavidin Sepharose™ High Performance for reproducibility and high performance.
  • Easy scale-up with HiTrap® Streptavidin HP prepacked columns.

Nota de análisis

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Información legal

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
SpinTrap is a trademark of Cytiva

Pictogramas

Flame
GHS02

Palabra de señalización

Warning

Frases de peligro

H226

Código de clase de almacenamiento

3 - Flammable liquids

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Certificados de análisis (COA)

Lot/Batch Number

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Protocolos

Purification or Removal of Biotin and Biotinylated Substances

This page shows how to purify or remove biotin and biotinylated substances with Streptavidin Sepharose High Performance from GE Healthcare.

Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules

Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules

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