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GE17-5280-01

nProtein A Sepharose™ 4 Fast Flow

Cytiva 17-5280-01, pack of 5 mL

Sinónimos:

Sepharose 4 Fast Flow

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About This Item

Código UNSPSC:
41106500
NACRES:
NA.56

ligand

native protein A (S. aureus)

envase

pack of 5 mL

fabricante / nombre comercial

Cytiva 17-5280-01

condiciones de almacenamiento

(20% Ehtanol)

matriz

4% cross-linked agarose

diámetro promedio

90 μm (d50v)

cleaning

2-10

working range

3-9

capacidad

>30 mg binding capacity(human IgG/ml)

idoneidad

suitable for bioprocess medium

temp. de almacenamiento

2-8°C

Categorías relacionadas

Descripción general

nProtein A Sepharose™ 4 Fast Flow is native protein A coupled to Sepharose™ 4 Fast Flow for the purification of monoclonal and polyclonal antibodies at both laboratory and process scale.

nProtein A Sepharose™ 4 Fast Flow is native protein A coupled to the well established Sepharose™ 4 Fast Flow base matrix. The native protein A ligand is produced by fermenting a selected strain of Staphylococcus aureus. The purified protein is coupled to the cross-linked 4% agarose base matrix by the cyanogen bromide technique, giving a highly stable medium with minimal non-specific adsorption. nProtein A Sepharose™ 4 Fast Flow is manufactured without using animal-derived components.

nProtein A Sepharose™ 4 Fast Flow has nearly twice the total IgG binding capacity of Protein A Sepharose™ CL-4B, and is suitable for recovery and purification of monoclonal antibodies from cell culture at both laboratory and process scale. nProtein A Sepharose™ 4 Fast Flow was developed and tested in co-operation with leading manufacturers of purified monoclonal antibody products, and is used in routine commercial production.

As member of the BioProcess media range, nProtein A Sepharose™ 4 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

Características y beneficios

  • Replaces Protein A Sepharose™ 4 Fast Flow, the first Cytiva Protein A medium for large-scale purification of antibodies.
  • Used in routine commercial production of monoclonal antibodies
  • Free from animal-derived components.

Almacenamiento y estabilidad

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Nota de análisis

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Información legal

Sepharose is a trademark of Cytiva

Pictogramas

Flame
GHS02

Palabra de señalización

Warning

Frases de peligro

H226

Código de clase de almacenamiento

3 - Flammable liquids

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Artículos

Purification Using Protein A-based Chromatography Media

This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.

Immunoprecipitation Techniques

This page describes immunoprecipitation (immunoaffinity or pull-down techniques).

Column Packing and Preparation for Affinity Chromatography of Antibodies

This page describes efficient column packing and preparation for affinity chromatography of antibodies.

Protocolos

Pull-down assays

This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from GE Healthcare.

Performing a Separation with GE Healthcare Products Based on Protein A

Protein A is derived from a strain of Staphylococcus aureus and contains five regions that bind to the Fc region of IgG. As an affinity ligand, protein A is coupled to Sepharose so that these regions are free to bind IgG.

Performing a Purification of IgG Antibodies with Protein G Sepharose® 4 Fast Flow

This page shows how to separate IgG antibodies by affinity chromatography using Protein G Sepharose 4 Fast Flow from GE Healthcare.

Performing a Purification of IgG Antibodies with Protein G Sepharose® 4 Fast Flow

This page shows how to separate IgG antibodies by affinity chromatography using Protein G Sepharose 4 Fast Flow from GE Healthcare.

Ver todo

Contenido relacionado

Protein Pull-Down

Pull-down assays, reagents, and protocols for investigating in vitro protein-protein interactions using affinity or GST pull-down, tandem affinity purification (TAP), and co-immunoprecipitation methods.

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