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Key Documents

GE17-5168-01

Sigma-Aldrich

Mono S® 5/50 GL

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About This Item

Código UNSPSC:
41115700
NACRES:
SB.52

ligand

-OCH2CH(OH)CH2OCH2CH(OH)CH2SO3-

Materiales

PEEK column

descripción

Ion Exchanger Type (Methyl sulfonate strong cation)

caducidad

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

envase

pkg of 1 mL

Parámetros

0.5 ml/min - 3.0 ml/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
580 psi

lecho tamaño

5 mm × 50 mm

lecho volumen

1 mL

columna D.I.

5.7 mm

matriz

monodisperse porous polystyrene/divinyl benzene beads
polymeric

grupo activo de la matriz

methyl sulfonate phase

tam. prom. pieza

10 μm

cleaning

1-14

working range

2-12

técnica de separación

cation exchange

Descripción general

Mono S® is a strong cation exchanger prepacked with MonoBeads in a Tricorn column. The column is an excellent choice for small-scale polishing in purification of proteins, peptides, and other biomolecules when high purity is required. The column has 1 mL bed volume for small-scale purifications.

The Tricorn columns offer high-resolution separations with greater loading capacity but slightly lower resolution than MiniBeads columns.

Características y beneficios

  • Monodisperse 10 μm porous beads yield high resolution, dynamic capacity, reproducibility, and durability.
  • Maximum resolution is achieved from the efficiency of small, perfectly spherical monodispersed particles optimally packed in columns coupled with the excellent selectivities of the Q ion exchangers.
  • Compatible with AKTA design, FPLC System, and other high-performance LC systems.
  • MonoBeads are also available as Mono P® for chromatofocusing

Almacenamiento y estabilidad

Store at 4 to 30 °C (0.2 M sodium acetate in 20% ethanol)
Store at 4 to 30 °C (20% Ethanol)

Nota de análisis

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

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Información legal

FPLC is a trademark of Cytiva
Mono P is a registered trademark of Cytiva
Mono S is a registered trademark of Cytiva

Pictogramas

Flame

Palabra de señalización

Warning

Frases de peligro

Código de clase de almacenamiento

3 - Flammable liquids


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Artículos

This page provides information about performing an isolation of recombinant protein complexes with different pull-down assays with products from GE Healthcare.

This page shows how to perform a purification of His-tagged membrane proteins with products from GE Healthcare.

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

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Protocolos

This page covers using Monobeads for purification of proteins, peptides, or oligonucleotides.

This page shows how to condition membrane proteins for further analysis with products from GE Healthcare.

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from GE Healthcare.

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

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