GE17-5079-02
HiTrap® rProtein A Fast Flow
Cytiva 17-5079-02, pack of 2 × 1 mL
About This Item
Productos recomendados
ligand
recombinant protein A (E. coli)
caducidad
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
envase
pack of 2 × 1 mL
fabricante / nombre comercial
Cytiva 17-5079-02
condiciones de almacenamiento
(20% Ehtanol)
(20% Ethanol)
Parámetros
<4 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
42 psi
lecho tamaño
7 mm × 25 mm
lecho volumen
1 mL
columna D.I.
7 mm
matriz
4% cross-linked agarose
tamaño de partícula
60-165 μm
diámetro promedio
90 μm (d50v)
cleaning
2-11(pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
working range
3-10
capacidad
~50 mg binding capacity (human lgG/ml medium)(Capacity of HiTrap rProtein A for some monoclonal antibodies. Running conditions: Binding buffer: 20 mM sodium phosphate (incl. 3 M NaCl for IgG), pH 7.0. Elution buffer: 0.1 M sodium citrate, pH 3.0.)
idoneidad
suitable for bioprocess medium
temp. de almacenamiento
2-8°C
Categorías relacionadas
Descripción general
Aplicación
Características y beneficios
- Quick, convenient preparative purification of monoclonal antibodies.
- Well-established Protein A-based medium used in many approved MAb processes.
- Recombinant Protein A exhibits similar Fc region specificity to that of native Protein A but shows enhanced binding capacity and fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
- Simple operations with a syringe, pump, or high-performance chromatography system, such as ÄKTA® design.
Nota de análisis
Información legal
Palabra de señalización
Warning
Frases de peligro
Consejos de prudencia
Código de clase de almacenamiento
3 - Flammable liquids
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Artículos
This page shows how to separate IgG antibodies by affinity chromatography using HiTrap® Protein G HP columns from GE Healthcare.
This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
Protocolos
Protein A is derived from a strain of Staphylococcus aureus and contains five regions that bind to the Fc region of IgG. As an affinity ligand, protein A is coupled to Sepharose so that these regions are free to bind IgG.
This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.
How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.
This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from GE Healthcare.
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