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GE17-1287-10

Anx Sepharose™ Fast Flow High Sub

Cytiva 17-1287-10, pack of 25 mL

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About This Item

Código UNSPSC:
47101511
NACRES:
NA.56

ligand

diethylaminopropyl

descripción

Ion Exchanger Type (value)

envase

pack of 25 mL

fabricante / nombre comercial

Cytiva 17-1287-10

matriz

4% cross-linked agarose

tamaño de partícula

45-165 μm

diámetro promedio

90 μm

cleaning in place

2-14

working range

3-13

idoneidad

suitable for bioprocess medium

Descripción general

ANX Sepharose™ 4 Fast FLow (high sub) is a weak anion exchanger, with a different selectivity compared with traditional ion exchangers. It is optimized for the separation of larger proteins.

Aplicación

ANX Sepharose™ Fast FLow (high sub) is part of the Sepharose™ Fast FLow ion exchange platform, which has been the industrial standard for ion exchange chromatography during recent decades. ANX Sepharose™ 4 Fast FLow (high sub) is a weak anion exchanger medium with tertiary amine groups attached to the base matrix Sepharose™ 4 Fast FLow via ether linkage and a hydrophilic spacer arm. The diethylaminopropylgroup is coupled in a mode that precludes formation of quaternary groups, which are typically seen on traditional DEAE media, resulting in a truly weak anion exchanger. The base matrix, Sepharose™ 4 Fast Flow, is a highly cross-linked, 4% Agarose derivative with high chemical and physical stability and broad separation range. ANX Sepharose™ 4 Fast FLow (high sub) has high chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols.

As member of the BioProcess media range, ANX Sepharose™ 4 Fast FLow (high sub) meets industrial demands with security of supply and comprehensive technical and regulatory support.

Características y beneficios

  • Weak anion exchanger with different selectivity compared with traditional ion exchangers.
  • Applicable for separation of high molecular weight Proteins
  • Developed in co-operation with leading large-scale pharmaceutical manufacturers
  • High chemical stability allows for well proven CIP and sanitization protocols
  • The hydrophilic nature of the base matrix ensures Low levels of non-specific binding leading to Low levels of host cell-derived impurities in the elution pool.

Almacenamiento y estabilidad

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Nota de análisis

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Información legal

Sepharose is a trademark of Cytiva

Pictogramas

Flame
GHS02

Palabra de señalización

Warning

Frases de peligro

H226

Código de clase de almacenamiento

3 - Flammable liquids

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Artículos

Non-volatile and Volatile Buffer Systems

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

Selection of Purification Equipment

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

Ion Exchange Chromatography Troubleshooting
Practical Considerations for IEX Separation

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

Protocolos

Sepharose Fast Flow: Purification with Good Resolution and Easy Scale-Up

This page covers the use of Sepharose Fast Flow for purification of proteins.

Column packing and preparation for Ion Exchange Chromatography & Chromatafocusing

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from GE Healthcare.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

Column Cleaning for Ion Exchange Chromatography and Chromatofocusing

This page covers detailed information on cleaning procedures and recommended flow for column cleaning.

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