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Key Documents

GE17-0444-01

Lentil Lectin-Sepharose™ 4B 25 mL

Cytiva 17-0444-01

Sinónimos:

Sepharose 4B

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About This Item

Código UNSPSC:
41106500
NACRES:
NA.56

ligand

lentil lectin

envase

pkg of 25 mL

fabricante / nombre comercial

Cytiva 17-0444-01

condiciones de almacenamiento

(20% Ehtanol, PBS pH 7)

matriz

4% agarose

tamaño de partícula

45-165 μm

diámetro promedio

90 μm

cleaning in place

3-12

working range

3-10

temp. de almacenamiento

2-8°C

Categorías relacionadas

Descripción general

Lentil Lectin Sepharose™ media is used for purification of a number of glycoproteins and other carbohydrate containing molecules. The lentil lectin ligand has more equal affinity for α-D-mannose and α-D-glucose compared to Con A.

Lentil lectin is a metalloprotein, containing Ca2+ and Mn2+, which binds reversibly to polysaccharides and glycoconjugates containing glucose or mannose type sugars. Lentil Lectin Sepharose™ 4B is a generally applicable group specific adsorbent and is routinely used in the preparation and purification of a number of glycoproteins and carbohydrate containing molecules.

Características y beneficios

  • Binds molecules that contain α-D-mannose, α-D-glucose, and sterically related residues
  • Compared to Con A, also for glycoprotein purification, Lentil Lectin distinguishes less sharply between glucosyl and mannosyl
  • Retains binding in 1% deoxycholate and is especially valuable for purification of detergent-solubilized membrane proteins
  • Lentil lectin is a metalloprotein, containing Ca2+ and Mn2+, which binds reversibly to polysaccharides and glycoconjugates containing glucose or mannose type sugars

Almacenamiento y estabilidad

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Nota de análisis

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Información legal

Sepharose is a trademark of Cytiva

Pictogramas

Flame
GHS02

Palabra de señalización

Warning

Frases de peligro

H226

Código de clase de almacenamiento

3 - Flammable liquids

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Artículos

Affinity Chromatography Troubleshooting

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Protocolos

Purification or Removal of Glycoproteins and Polysaccharides

Purification or Removal of Glycoproteins and Polysaccharides

Column Packing and Preparation for Affinity Chromatography with Specific Groups of Biomolecules

Column packing and preparation for Affinity Chromatography with specific groups of biomolecules.

Column Packing and Preparation Protocol for Affinity Chromatography

This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from GE Healthcare.

Sample Preparation for Affinity Chromatography

This page shows how to prepare samples for purification with affinity chromatography.

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