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Merck

G6795

Sigma-Aldrich

Anti-Green Fluorescent Protein (GFP), N-terminal antibody, Mouse monoclonal

clone GSN24, purified from hybridoma cell culture

Sinónimos:

Anti-GFP

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.56

origen biológico

mouse

conjugado

unconjugated

forma del anticuerpo

purified immunoglobulin

tipo de anticuerpo

primary antibodies

clon

GSN24, monoclonal

Formulario

buffered aqueous solution

mol peso

antigen 27 kDa

concentración

~2 mg/mL

técnicas

western blot: 1-2 μg/mL using GFP fusion proteins expressed in extracts of transfected cells

isotipo

IgG1

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C
2-8°C

modificación del objetivo postraduccional

unmodified

Descripción general

Anti-Green Fluorescent Protein (GFP), N-terminal antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma GSN24 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice.

Especificidad

The antibody reacts specifically with GFP fusion proteins.

Aplicación

Anti-Green Fluorescent Protein (GFP), N-terminal antibody, Mouse monoclonal has been used in: western blotting, enzyme linked immunosorbent assay (ELISA), immunofluorescence labeling.

Acciones bioquímicas o fisiológicas

Green Fluorescent Protein (GFP) is a unique tool in cellular and molecular biology research to monitor gene expression and protein localization in living cells. In the jellyfish, A. victoria, GFP transduces the excitation energy resulting from emission of blue light of the photoprotein aequorin and reemits it as green light.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Almacenamiento y estabilidad

For continuous use, store at 2–8 °C for up to one month. For extended storage, store at –20 °C in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Solutions at working dilution should be discarded if not used within 12 hours.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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