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Merck

G5154

Sigma-Aldrich

Glasgow Minimum Essential Medium

With sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Sinónimos:

BHK medium, GMEM, Glasgow′s MEM

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About This Item

Número MDL:
Código UNSPSC:
12352207
NACRES:
NA.75

product name

Glasgow Minimum Essential Medium, With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

esterilidad

sterile-filtered

formulario

liquid

técnicas

cell culture | mammalian: suitable

impurezas

endotoxin, tested

componentes

L-glutamine: no
phenol red: 0.0213 g/L
NaHCO3: 2.75 g/L
glucose: 4.5 g/L (Dextro)

Condiciones de envío

ambient

temp. de almacenamiento

2-8°C

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Descripción general

For use with adherent kidney cell lines such as baby hamster kidney cells (BHK).
Glasgow Minimum Essential Medium has been developed by modifying the Eagle′s BME medium. This medium can be used to study the genetic factors affecting cell competence.

Aplicación

Glasgow Minimum Essential Medium has been used to culture:
  • Hoxb7- green fluorescence protein (GFP) mouse embryonic stem (ES) cell line for the induction of ureteric bud differentiation
  • murine E14 Tg2a embryonic stem (mES) cells
  • transduced Chinese hamster ovary (CHO) cells for the expression and production of Siglec-1-Fc fusion proteins

Reconstitución

Supplement with 0.292 g/L L-glutamine and Tryptose phosphate broth solution (T 8159) at 100 ml/L of medium.

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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Marco D'Addio et al.
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Cellular interactions between endothelial cells and macrophages regulate macrophage localization and phenotype, but the mechanisms underlying these interactions are poorly understood. Here we explored the role of sialoglycans on lymphatic endothelial cells (LEC) in interactions with macrophage-expressed Siglec-1 (CD169). Lectin-binding
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The Ezh2 gene encodes a histone methyltransferase of the Polycomb Repressive Complex 2 that methylates histone H3 lysine 27. In this work we asked whether EZH2 has a role in the development of the pharyngeal apparatus and whether it regulates

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