G4045

Anti-GRP75 (SQ-15) antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• Sinónimos: Anti-Glucose Regulated Protein 75, Anti-Mitochondrial Hsp70, Anti-Mortalin, Anti-Peptide Binding Protein 74
• Número MDL: MFCD00164609
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• conjugado: unconjugated
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• formulario: buffered aqueous solution
• mol peso: antigen 75 kDa
• reactividad de especies: mouse, human, canine
• concentración: ~1 mg/mL
• técnicas: indirect immunofluorescence: 1-2 μg/mL using HeLa cells; western blot: 0.2-0.4 μg/mL using whole cell extracts of HeLa, MDCK, and NIH3T3 cells
• Nº de acceso UniProt: P38646
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: mouse ... Hspa9(15526) ; rat ... Hspa9(291671)

Descripción

Descripción general

GRP75 (glucose-regulated protein 75) belongs to a Hsp70 family of the heat shock proteins.

GRPs are unresponsive to heat stress and are induced by stress related to glucose starvation or defects in glycoprotein processing. GRP75/mortalin has been localized mainly to the endoplasmic reticulum (ER), but also to various cellular compartments including mitochondria and cytoplasmic vesicles.

Inmunógeno

synthetic peptide corresponding to amino acids 665-679 located at the C-terminus of human GRP75. This sequence is identical in mouse GRP75 (mot-1 and mot-2 isoforms) and highly conserved in rat (1 amino acid substitution).

Aplicación

Anti-GRP75 antibody produced in rabbit has been used in :

• immunofluorescence
• immunoblotting
• immunocytochemistry

Acciones bioquímicas o fisiológicas

GRP75 (glucose-regulated protein 75) is involved in stress responses to intracellular trafficking, antigen processing, and control of cell proliferation, differentiation, and tumorigenesis. It is induced by low levels of ionizing radiation, glucose deprivation, calcium ionophore, and ozone and its levels correlate with muscle activity, mitochondrial activity, and biogenesis.

Heat shock proteins (HSP) are considered to function as molecular chaperones by transiently binding to newly synthesized proteins to facilitate their correct folding and assembly. GRP75 levels are associated with muscle activity, mitochondrial function and biogenesis.

Descripción de destino

GRP75 (SQ-15) encodes a heat-shock cognate protein. This protein plays a role in the control of cell proliferation.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)