G115080

DMT-dG(dmf) Phosphoramidite

configured for PerkinElmer, configured for Polygen

• Sinónimos: DMT-dG(dmf) Amidite, N-[(dimethylamino)methylene]-5′-O-[bis(4-methoxyphenyl)phenylmethyl]-2′-deoxyguanosine, 3′-[2-cyanoethyl N,N-bis(1-methylethyl)phosphoramidite], N2-Dimethylformamidine-5′-O-(4,4′-dimethoxytrityl)-2′-deoxyguanosine-3′-O-[O-(2-cyanoethyl)-N,N′-diisopropylphosphoramidite]
• Fórmula empírica (notación de Hill): C₄₃H₅₃N₈O₇P
• Número de CAS: 330628-04-1
• Peso molecular: 824.90
• Número MDL: MFCD00797420
• Código UNSPSC: 12352200
• ID de la sustancia en PubChem: 329799827
• NACRES: NA.51

Propiedades

• tpo: for DNA synthesis
• Línea del producto: Proligo Reagents
• Análisis: ≥99% (³¹P-NMR); ≥99.0% (reversed phase HPLC)
• formulario: powder
• técnicas: oligo synthesis: suitable
• color: white to off-white
• λ: conforms (UV/VIS Identity)
• compatibilidad: configured for PerkinElmer; configured for Polygen
• perfil del nucleósido: base: deoxyguanosine; base protecting group: DMF; 2' protecting group: none; 5' protecting group: DMT; deprotection: fast/standard
• temp. de almacenamiento: -10 to -25°C
• cadena SMILES: COc1ccc(cc1)C(OC[C@H]2O[C@H](C[C@@H]2OP(OCCC#N)N(C(C)C)C(C)C)n3cnc4C(=O)NC(\N=C/N(C)C)=Nc34)(c5ccccc5)c6ccc(OC)cc6
• InChI: 1S/C43H53N8O7P/c1-29(2)51(30(3)4)59(56-24-12-23-44)58-36-25-38(50-28-45-39-40(50)47-42(48-41(39)52)46-27-49(5)6)57-37(36)26-55-43(31-13-10-9-11-14-31,32-15-19-34(53-7)20-16-32)33-17-21-35(54-8)22-18-33/h9-11,13-22,27-30,36-38H,12,24-26H2,1-8H3,(H,47,48,52)/b46-27-/t36-,37+,38+,59?/m0/s1
• Clave InChI: YRQAXTCBMPFGAN-UJASEYITSA-N

Descripción

Descripción general

Proligo′s DNA phosphoramidites lead to high-yield and high-quality oligonucleotides due to their high coupling efficiency. The deprotection step of automated oligonucleotide synthesis is integral to synthesis time and final product quality. The high coupling efficiency of Proligo′s DNA phosphoramidites leads to high-yield and high-quality oligonucleotides.Changing the dG protecting group to the dimethylformamide (dmf) base protecting group enables the rapid synthesis of high-purity, high-yieldoligonucleotide, thus increasing the efficiency of high-throughputproduction.Key Features of dG(dmf)

• dG(dmf) is deprotected faster than the conventional dG(ib): thedeprotection time in concentrated ammonia is reduced to 2 hours at55 °C or 1 hour at 65 °C
• The dG(dmf)-monomer is especially suitable for G-rich sequences:incomplete deprotection is greatly reduced in comparison with theconventional dG(ib)-monomer
• dG(dmf)-phosphoramidite is as stable in solution as the standard dA(bz)-,dC(bz)- and dT-phosphoramidites
• dG(dmf)-phosphoramidite can directly substitute for dG(ib)-phosphoramidite
• No change is required in the reagents commonly used for DNA synthesis(except a low concentration iodine oxidizer i.e., 0.02 M in iodine, should beemployed)DMT-dG(dmf) Phosphoramidite is configured for Expedite^™ and PolyGen^® Synthesizers.

Información legal

Expedite is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

PolyGen is a registered trademark of PolyGen GmbH.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable