G0251

β-Glucuronidase from bovine liver

Type B-1, ≥1,000,000 units/g solid

• Sinónimos: Bovine Liver beta-Glucuronidase, β-D-Glucuronide glucuronosohydrolase
• Número de CAS: 9001-45-0
• Comisión internacional de enzimas: 3.2.1.31 (BRENDA, IUBMB)
• Número CE: 232-606-8
• Número MDL: MFCD00130629
• Código UNSPSC: 12352204
• NACRES: NA.54

Propiedades

• tpo: Type B-1
• formulario: solid
• actividad específica: ≥1,000,000 units/g solid
• temp. de almacenamiento: −20°C

Descripción

Descripción general

β-Glucuronidase (GUSB) is a lysosomal enzyme, found in animals, plants and bacteria. This exoglycosidase shows high activity in spleen, liver and kidney. Bovine liver β-glucuronidase (BLG) is homotetramer with a molecular weight of 290 kDa.

Aplicación

β-Glucuronidase from bovine liver has been used in β-glucuronidase inhibition assay to study the β-glucuronidase inhibitory effect of extracts and isolated actives from Phyllanthus amarus. It has also been used to perform deglucuronidation.

β-glucuronidase is used as a reporter gene in GUS assays to monitor gene expression.

Acciones bioquímicas o fisiológicas

β-Glucuronidase (GUSB) deficiency can result in mucopolysaccharidosis type VII (MPSVII), which leads to store lysosome in the brain. Integration of β-glucuronides of steroid hormones into tissues can be done with the help of GUSB.

β-glucuronidase catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.

Definición de unidad

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay).

Información de seguridad

• Pictogramas: GHS08
• Palabra de señalización: Danger
• Frases de peligro: H317,H334
• Consejos de prudencia: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Clasificaciones de peligro: Resp. Sens. 1 - Skin Sens. 1
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)