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Merck

F8771

Sigma-Aldrich

Anti-Mouse IgG (Fab specific)−FITC antibody produced in goat

affinity isolated antibody, buffered aqueous solution

Sinónimos:

Goat Anti-Mouse IgG (Fab specific)−Fluorescein isothiocyanate

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About This Item

Número MDL:
Código UNSPSC:
12352203
NACRES:
NA.46

origen biológico

goat

Nivel de calidad

conjugado

FITC conjugate

forma del anticuerpo

affinity isolated antibody

tipo de anticuerpo

secondary antibodies

clon

polyclonal

Formulario

buffered aqueous solution

condiciones de almacenamiento

protect from light

técnicas

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200
indirect immunofluorescence: 1:64

temp. de almacenamiento

−20°C

modificación del objetivo postraduccional

unmodified

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Descripción general

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections.

Especificidad

Anti-Mouse IgG (Fab specific)-FITC antibody is specific for Fab fragment of all mouse IgG subclasses. It shows no reactivity with mouse IgG, Fc fragment, or human IgG. Goat anti-Mouse IgG is conjugated to FITC and then purified by gel filtration to remove free FITC. The antibody is adsorbed with human IgG and rat serum proteins to ensure minimal cross reactivity.

Inmunógeno

Purified mouse IgG

Aplicación

Anti-Mouse IgG (Fab specific)-FITC antibody may be used for immunofluorescence of human peripheral blood lymphocytes at a minimum dilution of 1:64. It may be used for immunohistochemistry of formalin-fixed, paraffin-embedded human tonsil slides at a working antibody dilution of 1:200. For immunofluorescence, a working dilution of 1:200 was used in various studies. To study DNA synthesis in mouse two-cell stage embryos by BrdU antibody, mouse IgG (Fab specific)-FITC antibody dilution of 1:50 was used.

Otras notas

Antibody adsorbed with human IgG and rat serum proteins.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Nota de preparación

Adsorbed to reduce background with human or rat samples

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

nwg

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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