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Merck

F7884

Sigma-Aldrich

Anti-Dog IgG (whole molecule)–FITC antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Sinónimos:

Rabbit Anti-Dog IgG (whole molecule)–Fluorescein isothiocyanate

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About This Item

Número MDL:
Código UNSPSC:
12352203
NACRES:
NA.46

origen biológico

rabbit

Nivel de calidad

conjugado

FITC conjugate

forma del anticuerpo

affinity isolated antibody

tipo de anticuerpo

secondary antibodies

clon

polyclonal

Formulario

buffered aqueous solution

condiciones de almacenamiento

protect from light

técnicas

direct immunofluorescence: 1:32

temp. de almacenamiento

−20°C

modificación del objetivo postraduccional

unmodified

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Descripción general

IgG (Immunoglobulin G) antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Rabbit anti-dog IgG is isolated by affinity isolation and conjugated to Fluorescein Isothiocyanate (FITC).
The variable region of IgG antibody is specific to antigens and is highly conserved. IgG has four subclasses- IgG1, IgG2a, IgG2b and IgG2c.

Especificidad

Anti-Dog IgG (whole molecule)-FITC antibody is specific for dog IgG subclasses.

Inmunógeno

Purified dog IgG

Aplicación

Anti-Dog IgG (whole molecule)-FITC antibody may be used for direct immunofluorescence using dog peripheral blood lymphocytes at a minimum working dilution of 1:32.

Acciones bioquímicas o fisiológicas

IgG (Immunoglobulin G) antibody provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. IgG antibody has its function similar to IgM antibody in complement system activation. IgG participates in hypersensitivity type II and type III. It helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Almacenamiento y estabilidad

For continuous use, store at 2-8 °C for up to one month.
For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

nwg

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Clinical cancer research : an official journal of the American Association for Cancer Research, 20(14), 3753-3762 (2014-05-31)
Due to the many similarities with its human counterpart, canine malignant melanoma (cMM) is a valuable model in which to assess the efficacy of novel therapeutic strategies. The model is herein used to evaluate the immunogenicity, safety, and therapeutic efficacy
Vanessa Figueredo Pereira et al.
Revista da Sociedade Brasileira de Medicina Tropical, 49(1), 104-106 (2016-05-11)
Conjunctival swab PCR was evaluated as a tool to diagnose visceral leishmaniasis in dogs. Conjunctival swab PCR was compared to indirect immunofluorescence antibody test and blood PCR. Indirect immunofluorescence was significantly correlated with conjunctival swab PCR (p < 0.05), but
Hanna D Bremer et al.
Scientific reports, 8(1), 4852-4852 (2018-03-21)
Dogs can spontaneously develop complex systemic autoimmune disorders, with similarities to human autoimmune disease. Autoantibodies directed at self-antigens are a key feature of these autoimmune diseases. Here we report the identification of interleukin enhancer-binding factors 2 and 3 (ILF2 and
Suckow M A, et al.
The Laboratory Rat, 205(3-4) (2014)
P R Romanelli et al.
Research in veterinary science, 82(2), 202-207 (2007-02-03)
Sheep and dog blood samples were collected from nine farms in the county of Guarapuava, Paraná, Brazil. The indirect fluorescent antibody test (IFAT) was used to detect Neospora caninum and Toxoplasma gondii antibodies. Herein, serum samples from 305 sheep were

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