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F7053

Sigma-Aldrich

Anti-vinculina monoclonal antibody produced in mouse

clone hVIN-1, purified from hybridoma cell culture

Sinónimos:

Monoclonal Anti-Vinculin antibody produced in mouse

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About This Item

Número MDL:
MFCD00162905
Código UNSPSC:
12352203
NACRES:
NA.41

origen biológico

mouse

Nivel de calidad

200

conjugado

FITC conjugate

forma del anticuerpo

purified immunoglobulin

tipo de anticuerpo

primary antibodies

clon

hVIN-1, monoclonal

formulario

buffered aqueous solution

mol peso

antigen 116 kDa

reactividad de especies

frog, chicken, mouse, canine, human, bovine, rat, turkey

condiciones de almacenamiento

protect from light

técnicas

direct immunofluorescence: 1:50 using methanol/acetone fixed cultured bovine MDBK cells

isotipo

IgG1

Nº de acceso UniProt

P18206

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... VCL(7414)
mouse ... Vcl(22330)
rat ... Vcl(305679)

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Especificidad

Marca de manera específica la vinculina en los contactos célula-célula y célula-sustrato. Reacciona fuertemente con la vinculina humana. Muestra reactividad cruzada con la metavinculina del músculo liso.

Inmunógeno

vinculina humana purificada del útero.

Aplicación

Monoclonal Anti-Vinculin-FITC antibody is suitable for immunofluorescence staining of fibroblasts cells to study the effects of nanofiber spatial organization on cell behaviors. It is also suitable for direct immunofluorescence at a dilution of 1:50 using methanol/acetone fixed cultured bovine MDBK cells.

Acciones bioquímicas o fisiológicas

Vinculin is a cytoskeletal protein with molecular mass of ~116 kDa. Vinculin is closely associated with adhesive membrane areas, such as focal contacts, podosomes, or intercellular junctions of the zonula adherens type. It acts as a mediator in interactions of integrins and the actin cytoskeleton. It forms a bridge between integrins, receptors for ECM molecules on the cell surface and the F-actin cytoskeleton. It is associated with talin and α-actinin which directly connects with integrins. It has ability to bind α-catenin as well as the vasodilator stimulated phosphoprotein (VASP) in focal contact assembly.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

nwg

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Focal adhesion sites are cell-matrix contacts that are regulated by phosphatidylinositol-4,5-bisphosphate (PIP2)-dependent pathways. Vinculin is a major structural component of these sites and is thought to be engaged in multiple ligand interactions at the cytoplasmic face of these contacts. Cytoplasmic
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