F1763
Anti-Rat IgG (whole molecule)–FITC antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
Sinónimos:
Rabbit Anti-Rat IgG (whole molecule)–Fluorescein isothiocyanate
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About This Item
Productos recomendados
origen biológico
rabbit
conjugado
FITC conjugate
forma del anticuerpo
affinity isolated antibody
tipo de anticuerpo
secondary antibodies
clon
polyclonal
Formulario
buffered aqueous solution
condiciones de almacenamiento
protect from light
técnicas
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:320
indirect immunofluorescence: 1:320
temp. de almacenamiento
−20°C
modificación del objetivo postraduccional
unmodified
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Descripción general
Anti-Rat IgG (whole molecule) is produced in rabbit using purified rat IgG as the immunogen.
Rat IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in rat serum. Fluorescein isothiocyanate (FITC) is a fluorescein derivative (fluorochrome) used to tag antibodies, including secondary antibodies, for use in fluorescence-based assays and procedures. FITC excites at 495 nm and emits at 521 nm.
Rat IgGs have four subclasses, namely, IgG1, IgG2a, IgG2b, and IgG2c. Rat IgGs against target proteins are often used as primary antibodies in various research applications. Anti-Rat IgG (whole molecule)-FITC antibody binds to all rat immunoglobulins.
Especificidad
Rabbit polyclonal anti-Rat IgG (whole molecule)−FITC antibody reacts with rat IgG in vitro, in rat serum and biological fluids.
Inmunógeno
Purified rat IgG
Aplicación
Anti-Rat IgG (whole molecule)-FITC antibody has been used:
- in immunolabelling for confocal laser scanning microscopy (CLSM)
- in immunostaining
Anti-Rat IgG (whole molecule)-FITC antibody is suitable for use in flow cytometry (1:100) and immunofluorescence.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Flow cytometry/Cell sorting (1 paper)
Immunofluorescence (1 paper)
Flow cytometry/Cell sorting (1 paper)
Immunofluorescence (1 paper)
Rabbit polyclonal anti-Rat IgG (whole molecule)-FITC antibody may be used to detect and quantitate the level of IgG in rat serum and biological fluids by fluorescent techniques. It may also be used as a secondary antibody in assays that use pig IgG as the primary antibody.
Acciones bioquímicas o fisiológicas
IgGs are glycoprotein antibodies that modulate several immune responses. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection. Thus, secondary anti-rat IgG is a useful tool for the analysis of target proteins.
Otras notas
Antibody adsorbed with human IgG.
Forma física
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Nota de preparación
Adsorbed to reduce background staining with human samples.
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
nwg
Equipo de protección personal
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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