F1680

Anti-Flotillin 2 (C-terminal)

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• Sinónimos: Anti-ECS, Anti-ESA, Anti-Epidermal surface antigen, Anti-FLOT2, Anti-Reggie-1
• Número MDL: MFCD00212936
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 200
• conjugado: unconjugated
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• formulario: buffered aqueous solution
• mol peso: antigen ~48 kDa
• reactividad de especies: human
• concentración: ~1 mg/mL
• técnicas: indirect immunofluorescence: 5-10 μg/mL using human HEK-293T cells; western blot: 0.5-1 μg/mL using whole extract of human HeLa cells
• Nº de acceso UniProt: Q14254
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... FLOT2(2319) ; mouse ... Flot2(14252) ; rat ... Flot2(83764)

Descripción

Descripción general

Flotillin-2 is a protein encoded by the FLOT2 gene in humans. Flotillin 2 is more widely distributed, but is absent in skeletal muscle. FLOT2, also called reggie-1, belongs to flotillin protein family integral membrane proteins comprising of prohibitin homology (PHB) domain or SPFH domain (stomatin/prohibitin/flotillin/HflK/C domain).

Inmunógeno

synthetic peptide corresponding to amino acid residues 415-428 of human flotillin 2 with an N-terminal added cysteine, conjugated to KLH. The corresponding sequence differs by 2 amino acids in rat and mouse.

Aplicación

Anti-Flotillin 2 (C-terminal) antibody produced in rabbit has been used in:

• immunofluorescence
• immunoprecipitation
• immunoblotting

Acciones bioquímicas o fisiológicas

Flotillin-2 is a protein encoded by the FLOT2 gene in humans. Flotillins are involved in cell adhesion processes and play a role during breast cancer progression. FLOT2 especially plays a functional role in cell-cell adhesion in non-malignant epithelial cells. It may have an essential function in intestinal physiology, especially in the colon. FLOT2 has been implicated in several signaling pathways in tumor cells and can be used as a prognostic biomarker for breast cancer progression.

The prohibitin homology (PHB) domain constitutes a lipid recognition motif which can target proteins to the plasma membrane. The association of flotillin 2 to the plasma membrane depends on palmitoylation and myristolation in a conserved Cys34. Flotillin 2 overexpression is associated with melanoma progression and transformation of SB2 melanoma cells to a highly metastatic line. It is also associated with expression of the thrombin receptor, protease activated receptor 1 (PAR-1), that is implicated in cell growth and metastasis. Flotillin 2 may be used as a marker protein for lipid raft microdomains.

Descripción de destino

Flotillin 2 (C-terminal) encodes a caveolae-associated, integral membrane protein, which is thought to function in neuronal signaling.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)