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Key Documents

F0926

Sigma-Aldrich

Suero fetal bovino

USDA approved, sterile-filtered, suitable for cell culture

Sinónimos:

FBS, FCS, Suero, sueros

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About This Item

Número MDL:
MFCD00132239
Código UNSPSC:
12352207
NACRES:
NA.71

origen biológico

bovine fetus

Nivel de calidad

500

esterilidad

sterile-filtered

Línea del producto

FBS Classic

calidad

USDA approved

composición

Hemoglobin, ≤25 mg/dL

técnicas

cell culture | mammalian: suitable

impurezas

≤10 EU/mL endotoxin

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

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Descripción general

Fetal bovine serum (FBS) or fetal calf serum is the blood fraction remaining after the natural coagulation of blood. It comes from the blood drawn from a bovine fetus via a closed system of collection at the slaughterhouse. It is the most widely used serum-supplement for the in vitro cell culture of eukaryotic cells.

Aplicación

FBS sourced from USDA approved non-USA countries is used in a broad range of cell culture applications. FBS provides many non-defined growth promoting and survival enhancing factors to cells in culture.

Fetal Bovine Serum has been used for the following cell cultures:
  • Primary Culture Human Macrophages (mononuclear cells were re-suspended in Roswell Park Memorial Institute (RPMI) medium containing fetal bovine serum (FBS) along with other components).
  • Tissue Culture (MEFs were cultured in DMEM supplemented with FBS along with components).
  • Isolation and 3D culture of primary mammary epithelial organoids
  • Cell culture and medium (HUVECs were grown in Endothelial Cell Growth Medium-2 supplemented with FBS and growth factors)

Idoneidad

USDA Approved Sourcing refers to FBS produced from blood collected in countries outside of the United States that have been approved by the USDA (United States Department of Agriculture) for export of beef products into the United States. Eligible countries for export into the U.S. include Australia, Canada, Chile, Costa Rica, Honduras, Iceland, Japan, Mexico, New Zealand, Nicaragua, Uruguay. Foreign serum is tested to ensure the absence of exotic viruses before release by the USDA.

Nota de análisis

  • Analizada la presencia de endotoxinas y hemoglobina
  • Tested for the presence of bacteria, virus, and mycoplasma
  • Triple filtered with 0.1 micron membrane under aseptic conditions

Otras notas

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Cultured cells are excellent hosts for the propagation of many types of viruses. The ability of cell culture systems to produce large quantities of attenuated viral particles has served as the basis for the production of both human and veterinary vaccines. Traditional methods have relied on the production of viral agents in cells cultured in medium supplemented with serum, most commonly fetal bovine serum (FBS).

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Review the basics of using fetal bovine (or calf) serum for cell culture and how sourcing and regulations affect the price of sera for cell-based assays.

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Find answers to frequently asked questions (FAQs) about FBS and other serum products, and links to related products.

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