E6779
EZview™ Red Anti-HA Affinity Gel
Sinónimos:
Anti-HA
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About This Item
Código UNSPSC:
12352203
NACRES:
NA.56
Productos recomendados
caducidad
1 yr at 2‑8 °C
clases químicas de analitos
proteins (hemagglutinin)
técnicas
affinity chromatography: suitable
immunoprecipitation (IP): suitable
Matriz
crosslinked agarose
capacidad
≥0.4 mg/mL, gel binding capacity (HA-tagged fusion protein)
temp. de almacenamiento
2-8°C
Descripción general
EZview Red Anti-HA Affinity Gel is a red colored Anti-HA agarose affinity gel that contains anti-HA monoclonal antibody which is covalently attached to crosslinked agarose beads. The HA tag has the sequence YPYDVPDYA and derives from the hemagglutinin (HA) protein. This sequence occurs at amino acid residues 98-106 of the full-length HA protein. The HA tag has a high presence of charged amino acids, which enhances its likelihood of strong antibody recognition.
Especificidad
Binding specificity: HA-tag peptide (N-Tyr-Pro-Tyr-Asp-Val-Pro-Asp-Tyr-Ala-C). It recognizes reduced forms of HA-tagged proteins and is reactive with N or C-terminal HA-tagged fusion proteins.
Aplicación
EZview™ Red Anti-HA Affinity Gel has been used for immunoblot analysis and immunoprecipitation assay.
Suitable for Affinity capture (immunoprecipitation) of N- or C- terminal HA-tagged fusion proteins from E. coli or mammalian cell.
When performing small-scale affinity capture, such as immunoprecipitation, the affinity matrix is difficult to see in the microcentrifuge tubes. Accidental aspiration of the resin leads to quantitative variability in results. The EZview™ Red Affinity Gels greatly reduces the risk of pellet loss. EZview resins perform as well as conventional non-colored affinity gels, but allow the user to easily differentiate pellet from supernatant. This correlates to more accurate data because less protein is lost.
Características y beneficios
- Increased visibility - Red color reduces risk of incidental aspiration
- Improved recovery of target protein by reduced accidental loss
- Higher reproducibility - More consistent yields
Otras notas
The affinity gel contains affinity purified anti-HA mouse monoclonal antibody (Clone HA-7) covalently attached to beaded agarose. The HA tag corresponds to amino acid residues 98-106 (YPYDVPDYA) of human influenza virus hemagglutinin (HA).
Forma física
1:1 (v/v) suspension in PBS containing 50% glycerol and 15 ppm Kathon
Información legal
EZview is a trademark of Sigma-Aldrich Co. LLC
Producto relacionado
Referencia del producto
Descripción
Precios
Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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Contenido relacionado
Protein expression technologies for expressing recombinant proteins in E. coli, insect, yeast, and mammalian expression systems for fundamental research and the support of therapeutics and vaccine production.
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