Saltar al contenido
Merck

E1281

Sigma-Aldrich

Anti-Ezrin antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Sinónimos:

Anti-80K, Anti-Cytovillin, Anti-p81

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización


About This Item

Número MDL:
Código UNSPSC:
12352203
NACRES:
NA.41

origen biológico

rabbit

conjugado

unconjugated

forma del anticuerpo

affinity isolated antibody

tipo de anticuerpo

primary antibodies

clon

polyclonal

formulario

buffered aqueous solution

mol peso

antigen ~80 kDa

reactividad de especies

human, rat

técnicas

immunohistochemistry: 20-40 μg/mL
immunoprecipitation (IP): 0.5-1 μg using RIPA extract of human A431 cells
indirect immunofluorescence: 10-20 μg/mL
western blot (chemiluminescent): 0.5-1 μg/mL using whole extract of rat NRK cells

Nº de acceso UniProt

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... EZR(7430)
mouse ... Ezr(22350)
rat ... Ezr(54319)

Descripción general

Ezrin is a 80kDa membrane-associated protein that belongs to ERM (Ezrin/Radixin/Moesin) family. Ezrin is found predominantly in polarized epithelial cells such as intestinal epithelial cells, gastric parietal cells, epithelial cells of the kidney proximal tubule, terminal bronchiole of the lung, and in mesothelia.

Inmunógeno

synthetic peptide corresponding to amino acids 479-498 of human ezrin with N-terminal added cysteine, conjugated to KLH. The corresponding sequence in rat and mouse differs by three amino acids.

Aplicación

Anti-Ezrin antibody produced in rabbit has been used in:
  • immunolocalization studies
  • immunostaining
  • immunofluorescence and immunohistochemistry
  • immunoblot

Acciones bioquímicas o fisiológicas

Cultured neuronal cells do express ezrin to some extent, and whole brain tissue expresses low levels of ezrin. Ezrin expression is associated with malignancy in astrocytoma, osteosarcoma, mammary and pancreatic adenocarcinomas, and in cutaneous and uveal melanomas.
Ezrin promotes cell adhesion. The NH2-terminal domain negatively regulates membrane spreading and elongation properties of ezrin COOH-terminal domain. The members of ERM family are usually involved in cross-linking of plasma membrane and actin filaments. ERM proteins are involved in cell adhesion, cell shape determination, migration, and the organization of cell surface structures. They are also involved in signal transduction and apoptosis. Ezrin has been implicated in tumor progression and metastatic spread.

Forma física

Solution in 0.01 M phosphate buffered saline containing 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

¿No encuentra el producto adecuado?  

Pruebe nuestro Herramienta de selección de productos.

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

nwg

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Vijay B Arumugham et al.
Frontiers in immunology, 9, 919-919 (2018-05-17)
A central feature of the immune synapse (IS) is the tight compartmentalization of membrane receptors and signaling mediators that is functional for its ability to coordinate T cell activation. Second messengers centrally implicated in this process, such as Ca2+ and
AICAR induces mitochondrial apoptosis in human osteosarcoma cells through an AMPK-dependent pathway
Morishita M, et al.
International journal of oncology, 50(1), 23-30 (2017)
Heat shock protein family B member 1 facilitates ezrin activation to control cell migration in esophageal squamous cell carcinoma
Xie YH, et al.
The International Journal of Biochemistry & Cell Biology, 112(19), 79-87 (2019)
Suvi Ilmonen et al.
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 18(4), 503-510 (2004-10-12)
Ezrin is a member of the ezrin-radixin-moesin family of proteins that link the actin-containing cytoskeleton to the plasma membrane. Ezrin is also connected to signaling molecules involved in the regulation of cell survival, proliferation and migration. Here, we examined the
A Bretscher
Current opinion in cell biology, 11(1), 109-116 (1999-02-27)
Molecules involved in ERM (ezrin-radixin-moesin) based attachment of membrane proteins to the cortical cytoskeleton in cell surface structures have been identified. In lymphocytes, a direct interaction is seen with extracellular matrix receptors and intercellular adhesion molecules. In polarized epithelial cells

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico