DUO96040
Duolink® PLA Multicolor Probemaker Kit - Far Red
Proximity Ligation Assay Multiplexing: protein-protein interaction, post-translational modification and low abundant protein events
Sinónimos:
in situ Proximity Ligation Assay, Flowcytometry-PLA, Protein Protein Interaction Kit
About This Item
Productos recomendados
técnicas
immunofluorescence: suitable
multiplexing: suitable
proximity ligation assay: suitable
fluorescencia
λex 646 nm; λem 664 nm (Far Red)
λex 646 nm; λem 664 nm
idoneidad
suitable for fluorescence
Condiciones de envío
dry ice
temp. de almacenamiento
−20°C
Aplicación
Far Red fluorescence detection reagents are often used with Cy®5 filter.
Application Note
Primary antibodies are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC), or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink® in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.
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Características y beneficios
- Detect multiple PPI, PTM and low abundant protein events in a single sample.
- Visualize protein complex formation and dissociation
- Monitor protein signaling pathways
- No overexpression or genetic manipulation required
- High specificity (fewer false positives)
- Detection of low abundant proteins due to rolling circle amplification
- Relative quantification possible
- No special equipment needed
- Faster and more simple than FRET
- Increased accuracy compared to co-IP
- Publication-ready results
Components
Duolink® PLA Multicolor Probemaker Kit - Far Red contains all the necessary reagents to perform the conjugations of Far Red Oligo H and Far Red Oligo I directly to primary antibodies. The resulting PLA probes (Far Red H and Far Red I) must be used together and cannot be paired with any other Duolink® PLA probe.
This product is comprised of the following:
- Far Red Oligo H DUO86040H
- Far Red Oligo I DUO86040I
- Conjugation Buffer DUO82033
- Stop Reagent DUO82034
- Storage Solution DUO82035
See datasheet for more information.
To perform a Multicolor Duolink® PLA experiment primary antibodies (PLA, IHC, ICC or IF validated) that recognize protein targets of interest are required. Duolink® PLA Multicolor Probemaker-generated probes (Red A/Red B, Green C/Green D, Orange F/Orange G, and/or FarRed H/FarRed I) are used in combination to perform the Multiplex experiment.The reagents in the Duolink® PLA Multicolor Reagent Pack are required to perform the amplification and detection of the resulting PLA probes. Required additional reagents include Duolink® Wash Buffers and Mounting Medium.
Información legal
Palabra de señalización
Warning
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Aquatic Chronic 2 - Skin Sens. 1
Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 3
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Artículos
Duolink® PLA Multicolor technology and the quantification of protein-protein interactions in fixed tissues and cells.
Protocolos
Duolink® PLA Multicolor Detection Protocol
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