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Merck

DUO92104

Sigma-Aldrich

Duolink® In Situ Orange Starter Kit Mouse/Goat

Sinónimos:

in situ Proximity Ligation Assay reagent, Protein Protein Interaction Kit

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About This Item

Código UNSPSC:
41116158
NACRES:
NA.32

Línea del producto

Duolink®

Nivel de calidad

técnicas

proximity ligation assay: suitable

fluorescencia

λex 554 nm; λem 576 nm (orange) (Cyanine 3; Zeiss Filter set 20)

idoneidad

suitable for fluorescence

Aplicación

Duolink®proximity ligation assay(PLA®) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.

Follow the Duolink® In Situ Fluorescence Protocol to use this product. A set of short instructionsis also available.

Visit our Duolink® PLA Resource Center for information on how to run a Duolink® experiment, applications, troubleshooting, and more.

To perform a complete Duolink® PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. This starter kit supplies all other necessary reagents for 30 Duolink® PLA reactions, which include a pair of PLA probes (Anti-Mouse PLUS and Anti-Goat MINUS), orange detection reagents, wash buffers, and mounting medium.Note that the primary antibodies must come from the same species as the Duolink® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.
Specificity
The Duolink® In Situ Orange Starter Kit Mouse/Goat requires one primary antibody from mouse and one primary antibody from goat. Orange fluorescence detection reagents are often used with Cyanine 3 filter.

Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink® PLA in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.

Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink® projects

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Características y beneficios

  • No overexpression or genetic manipulation required
  • High specificity (fewer false positives)
  • Single molecule sensitivity due to rolling circle amplification
  • Relative quantification possible
  • No special equipment needed
  • Quicker and simpler than FRET
  • Increased accuracy compared to co-IP
  • Publication-ready results

Información legal

Duolink is a registered trademark of Merck KGaA, Darmstadt, Germany
PLA is a registered trademark of Merck KGaA, Darmstadt, Germany

Los componentes del kit también están disponibles por separado

Referencia del producto
Descripción
SDS

  • DUO92006Duolink® In Situ PLA® Probe Anti-Goat MINUS, Affinity purified Donkey anti-Goat IgG (H+L)SDS

  • DUO92001Duolink® In Situ PLA® Probe Anti-Mouse PLUSSDS

  • DUO92007Duolink® In Situ Detection Reagents OrangeSDS

  • DUO82049Duolink® In Situ Wash Buffers, FluorescenceSDS

  • DUO82040Duolink® In Situ Mounting Medium with DAPISDS

Palabra de señalización

Danger

Frases de peligro

Clasificaciones de peligro

Aquatic Chronic 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Sens. 1

Código de clase de almacenamiento

8A - Combustible corrosive hazardous materials

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Shaida Ouladan et al.
International journal of oncology, 46(6), 2595-2605 (2015-04-23)
Solitary fibrous tumors (SFTs) are rare mesenchymal neoplasms, displaying variable morphological and clinicopathological features. Supportive immunohistochemical markers such as CD34, CD99, BCL2 and LSD1 are commonly applied in the differential diagnosis of SFTs, although none is sufficiently sensitive or specific
Linbo Wang et al.
Journal of cancer research and clinical oncology, 140(12), 1997-2008 (2014-07-10)
Our previous work identified leucine zipper transcription factor-like 1 (LZTFL1) as a novel tumor suppressor gene, with its expression correlated with survival outcome in gastric cancer (GC) patients. This study focuses on the role of LZTFL1 in GC aggression and
Gunisha Sagar et al.
Gut, 65(7), 1165-1174 (2015-06-11)
New-onset diabetes and concomitant weight loss occurring several months before the clinical presentation of pancreatic cancer (PC) appear to be paraneoplastic phenomena caused by tumour-secreted products. Our recent findings have shown exosomal adrenomedullin (AM) is important in development of diabetes
Dipti Pravin Lambade et al.
Journal of clinical and diagnostic research : JCDR, 9(2), ZC01-ZC05 (2015-04-11)
The purpose of this vitro study was to comparatively evaluate the adhesive bonding of dual cured resin luting agents with lithium disilicate ceramic material. Porcelain laminate veneers were prepared with lithium disilicate ceramic material i.e. IPS Empress II( E-Max Press).
Seo-Young An et al.
Journal of prosthodontics : official journal of the American College of Prosthodontists, 24(8), 642-646 (2015-04-14)
This study examined the radiopacity of contemporary luting cements using direct digital radiography under a range of exposure conditions. Disc specimens (N = 80, n = 10 per group, ø5 mm × 1 mm) were prepared from 8 resin-based luting

Artículos

Duolink proximity ligation assay used to study neuron interactions furthering neuroscience research.

Protocol for immunofluorescent detection of proteins in cells and tissue

Find Duolink references based on the type of method used, post translational modification detected, and research focus.

Things to consider for preparation, setup and execution of the Duolink® assay protocol

Ver todo

Protocolos

This page details the Duolink® In Situ Short Protocol for fluorescence detection

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